FastQCFastQC Report
Thu 26 May 2016
ERR580013_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580013_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6979624
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122180.17505240970000674No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98190.14068093066331366No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111400.047.2738881
GTATCAA187000.038.9994661
CAACGCA214800.032.4811975
ATCAACG218900.031.9397773
TATCAAC231300.031.0430472
AACGCAG240050.029.873286
TCAACGC241000.029.147014
ACGCAGA263000.026.407487
GTACTAG38250.025.9381791
CGCAGAG270100.025.661318
GTGGTAT44800.023.8251881
GTACATG292550.023.0000081
TACATGG294450.022.580772
GCAGAGT302550.022.3841429
GTATAAG43750.022.0325241
GCATCGT37200.021.9794948
ACATGGG298550.021.2452343
GAGTACT121050.020.9316512-13
TAGGCAT52450.020.8854035
GGGCTAT46800.020.6848076
Produced by FastQC (version 0.11.5)