FastQCFastQC Report
Thu 26 May 2016
ERR580013_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580013_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6979624
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112120.16063902582717923No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91890.1316546564686006No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107900.046.9978871
GTATCAA182750.039.1579131
CAACGCA208750.032.6673855
ATCAACG213450.031.9274333
TATCAAC227700.031.0017762
AACGCAG237600.029.3916556
TCAACGC234900.029.271444
ACGCAGA261250.026.0293877
GTACTAG38550.025.9419611
CGCAGAG268900.025.0267188
TAGGCAT53750.022.1215465
GTACATG285750.021.7910941
GCAGAGT303050.021.7721549
TACATGG286350.021.2381532
GTGGTAT47700.020.7678811
ACATGGG288700.020.3170173
TAGTACT53750.020.110934
GAGTACT121300.020.0681912-13
CTAGGCA61300.020.0107274
CGACGAG22600.019.4453424-25
Produced by FastQC (version 0.11.5)