FastQCFastQC Report
Thu 26 May 2016
ERR580012_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580012_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5433136
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG64500.037.1072771
GGGCACC74200.035.41317494
GCATCGT65850.034.2463578
TAGGCAT79300.033.6685265
CAACGCA100750.033.358765
TAGTACT89200.033.147194
CTGTGCG91450.032.781289
GTATCAA114800.032.6450581
CTAGGCA86850.031.3920084
GGTATCA75450.030.4754831
GTACATG258500.030.2323191
ATCAACG111300.030.1125343
GTACTGT100600.029.8884186
TACATGG262350.029.4068472
CTAGTAC103750.029.3135973
ACTGTGC108300.029.2388538
ACATGGG259550.028.8682353
AGGCATC79550.028.5842066
TACTGTG112750.028.2523177
CTAACGC30550.027.8496843
Produced by FastQC (version 0.11.5)