FastQCFastQC Report
Thu 26 May 2016
ERR580012_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580012_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5433136
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG63400.040.043611
GGGCACC73850.038.1207294
TAGGCAT78400.034.950815
TAGTACT92000.033.7182354
CTGTGCG95350.033.4664659
CAACGCA101500.032.4143075
CTAGGCA86450.032.2402534
AGGCATC75650.031.4971876
GTATCAA118650.030.6295381
GTACATG254650.030.6288991
GTACTGT106300.030.2851736
GGTATCA77700.030.1931341
GCATCGT79200.030.0260438
TACTGTG113600.029.9938687
ACTGTGC113000.029.82048
TACATGG258850.029.7315252
CTAGTAC108150.029.5960223
ACATGGG251100.029.5187323
ATCAACG112000.029.4599363
CATGGGG207550.027.4918984
Produced by FastQC (version 0.11.5)