FastQCFastQC Report
Mon 6 Jun 2016
ERR523206_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523206_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7521617
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT298050.396257879123598No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239770.31877454010221473No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152120.20224374625828465No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110950.14750817543621272No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG155650.059.2177542
GTACCTG177450.052.385311
ACCTGGG176550.051.5682753
CCTGGGG150100.041.821464
GGTATCA188450.036.246751
GTATCAA295450.033.8037261
TATAACG8750.031.7094652
CTGGGGG94300.029.6660985
GTACCCG15150.028.2570821
TCAACGC352050.027.8350474
TGGGGGG152300.027.6454016
TATCAAC359350.027.3771972
CAACGCA359100.027.2989435
GTACATG324900.027.293561
ATCAACG359550.027.2834913
TAACGCA9850.027.2105454
TATCACG7100.027.156292
AACGCAG371200.026.5463776
ATAACGC10050.026.2039593
TACATGG336900.026.1725032
Produced by FastQC (version 0.11.5)