##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR523206_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7521617
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.44866349881947	33.0	31.0	34.0	28.0	34.0
2	31.783719112525937	34.0	31.0	34.0	28.0	35.0
3	31.778206202203595	34.0	31.0	34.0	27.0	36.0
4	34.077685955028024	35.0	34.0	37.0	32.0	37.0
5	34.16786071399275	35.0	34.0	37.0	31.0	37.0
6	34.39502104401221	35.0	35.0	37.0	30.0	37.0
7	34.282114204964174	35.0	34.0	37.0	31.0	37.0
8	34.389548417580954	36.0	34.0	37.0	30.0	37.0
9	35.563296030627455	37.0	35.0	39.0	31.0	39.0
10-11	35.52145343215428	37.0	34.5	39.0	30.0	39.0
12-13	35.55011754786238	37.5	35.0	39.0	30.0	39.0
14-15	36.327577766855185	38.0	35.0	41.0	30.5	41.0
16-17	36.48587597587061	38.0	36.0	41.0	30.5	41.0
18-19	36.344521530410276	38.0	35.5	41.0	29.5	41.0
20-21	36.44389544163177	38.0	35.5	41.0	30.0	41.0
22-23	36.286023404275966	38.0	35.5	40.0	30.0	41.0
24-25	36.26848522066465	38.0	35.0	40.0	30.0	41.0
26-27	36.1524777185544	38.0	36.0	40.0	29.5	41.0
28-29	35.91230063960981	38.0	35.0	40.0	29.0	41.0
30-31	35.716044568608055	37.0	35.5	40.0	28.5	41.0
32-33	35.67856632423587	37.5	34.5	40.0	27.5	41.0
34-35	35.521399122023894	37.5	34.5	40.0	27.5	41.0
36-37	35.33576237662726	37.5	34.5	40.0	27.5	41.0
38-39	35.164172211906035	36.5	34.5	40.0	27.0	41.0
40-41	35.10166577213384	37.0	34.0	40.0	26.5	41.0
42-43	34.88489955018981	36.0	35.0	40.0	26.0	41.0
44-45	34.80416385200151	37.0	33.5	40.0	26.0	41.0
46-47	34.56688010038267	36.5	33.5	40.0	24.0	41.0
48-49	34.41993503524574	37.0	33.0	40.0	23.0	41.0
50-51	33.37025608988067	36.5	32.0	38.5	22.0	40.0
52-53	33.57832212142682	36.5	32.5	38.5	22.5	39.5
54-55	34.42463595261498	37.0	33.0	39.5	24.0	41.0
56-57	34.555461013236915	37.0	34.0	40.0	24.5	41.0
58-59	34.48498361456054	37.0	33.5	40.0	23.5	41.0
60-61	34.22697805538357	36.5	34.0	39.5	23.5	41.0
62-63	34.04591599120242	36.5	33.0	39.0	23.5	41.0
64-65	33.6363137740196	36.0	33.0	39.0	22.5	41.0
66-67	33.43801651958615	36.0	33.0	38.0	22.5	40.5
68-69	33.0977601624757	35.0	32.5	37.5	21.5	40.0
70-71	32.889783340470544	35.0	32.0	37.0	21.0	40.0
72-73	32.56001887626025	35.0	32.0	37.0	20.5	39.0
74-75	32.179304795764	35.0	32.0	36.5	20.0	39.0
76-77	32.061558638255576	35.0	31.0	37.0	19.0	38.5
78-79	31.789328877022058	35.0	31.0	36.0	19.0	38.0
80-81	31.535166108564155	35.0	31.0	36.0	18.5	37.5
82-83	31.223788515155718	34.5	30.5	35.5	18.0	37.0
84-85	30.9861521797773	35.0	30.0	35.0	17.5	37.0
86-87	30.742974150372188	34.0	30.0	35.0	17.5	37.0
88-89	30.590080297893394	34.0	30.0	35.0	17.5	36.5
90-91	30.38169312529473	34.0	29.5	35.0	17.0	36.0
92-93	30.12310890065261	34.0	29.0	35.0	16.5	36.0
94-95	30.018340870054935	34.0	29.0	35.0	16.0	36.0
96-97	29.821213111489193	34.0	29.0	35.0	15.5	36.0
98-99	29.723541427328726	34.0	28.5	35.0	15.0	36.0
100	28.47759943107978	33.0	25.0	35.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.09510734115994879
1101	2	0.05559625556097458
1101	3	0.10700528675181786
1101	4	0.06080484691810284
1101	5	-0.016021319574036852
1101	6	0.0415006401379685
1101	7	0.04049731210991325
1101	8	0.14373282063115056
1101	9	0.15807184633132465
1101	10-11	0.06617017847394635
1101	12-13	0.03539600687375355
1101	14-15	-0.008894651705460888
1101	16-17	0.022178474078891952
1101	18-19	0.0685532232996664
1101	20-21	-0.019329006368678847
1101	22-23	0.031842167089315865
1101	24-25	-0.02505515297081473
1101	26-27	-0.001321668920432728
1101	28-29	-0.09472789097691958
1101	30-31	-0.1509441736074706
1101	32-33	-0.12848146304171593
1101	34-35	-0.24396551960843027
1101	36-37	-0.21989872639309738
1101	38-39	-0.24815004337355617
1101	40-41	-0.30921159258445385
1101	42-43	-0.2391308800776173
1101	44-45	-0.1389872760988382
1101	46-47	-0.16811407273578283
1101	48-49	-0.25447844887828097
1101	50-51	-0.2857053622368113
1101	52-53	-0.17461350200623116
1101	54-55	-0.0402204072773813
1101	56-57	-0.05825005470615707
1101	58-59	-0.17670316190514512
1101	60-61	-0.10485479078562321
1101	62-63	-0.20420847825780442
1101	64-65	-0.17221772184804252
1101	66-67	-0.08260646538171557
1101	68-69	-0.10918319664509823
1101	70-71	-0.18519040143166876
1101	72-73	-0.14502191097914618
1101	74-75	-0.1338593691932104
1101	76-77	-0.17078316989800157
1101	78-79	-0.3273378291654865
1101	80-81	-0.2938514746051446
1101	82-83	-0.4317645423574312
1101	84-85	-0.3172478546627211
1101	86-87	-0.26765130648589874
1101	88-89	-0.42518551554287143
1101	90-91	-0.41490188277290585
1101	92-93	-0.3571330998199471
1101	94-95	-0.4039219519453461
1101	96-97	-0.4662472328174694
1101	98-99	-0.41942558219252035
1101	100	-0.4176458782117365
1102	1	0.003720450681232279
1102	2	-0.0445773958107516
1102	3	0.020172293703364375
1102	4	-0.0851188711085129
1102	5	-0.06329907022497139
1102	6	0.017036627767531343
1102	7	0.012028887831419866
1102	8	0.00459651365160596
1102	9	-0.13216370617573858
1102	10-11	-0.07231801452358866
1102	12-13	-0.07202153209284035
1102	14-15	-0.11841289526277876
1102	16-17	-0.1334064561156012
1102	18-19	-0.036781322655564
1102	20-21	-0.19044853100546533
1102	22-23	-0.19123066841473246
1102	24-25	-0.1686170365243953
1102	26-27	-0.25563359464796775
1102	28-29	-0.14302568023055784
1102	30-31	-0.2491060677546102
1102	32-33	-0.15395479433645676
1102	34-35	-0.19791122180374288
1102	36-37	-0.6552990882063554
1102	38-39	-0.32892487879125554
1102	40-41	-1.0828769332751236
1102	42-43	-1.0150642487821813
1102	44-45	-0.4531474074709152
1102	46-47	-0.4141802104067196
1102	48-49	-0.43573906748014224
1102	50-51	-0.4963244166172558
1102	52-53	-0.3263639772700273
1102	54-55	-0.24446262542142705
1102	56-57	-0.07366276380850678
1102	58-59	-0.10355459873523643
1102	60-61	-0.23317557282214096
1102	62-63	-0.47471924756573003
1102	64-65	-0.27538077253350934
1102	66-67	-0.28557721468055774
1102	68-69	-0.2720899453837049
1102	70-71	-0.3419506386503599
1102	72-73	-0.2271885797971649
1102	74-75	-0.19155117613929207
1102	76-77	-0.29186930032555836
1102	78-79	-0.18130492991607028
1102	80-81	-0.2683188327021959
1102	82-83	-0.41384630316215976
1102	84-85	-0.5707594581025752
1102	86-87	-0.512694697815288
1102	88-89	-0.5288537635290211
1102	90-91	-0.4889258059793562
1102	92-93	-0.509433895182994
1102	94-95	-0.6667719160622418
1102	96-97	-0.7115053329996499
1102	98-99	-0.660436297271481
1102	100	-0.8615825907141144
1103	1	-0.004175387656111695
1103	2	6.367573055143794E-4
1103	3	-0.013336789859454967
1103	4	0.05248670657331189
1103	5	0.18458325029622813
1103	6	0.15504247059219267
1103	7	0.08330721519717343
1103	8	0.06572925450986133
1103	9	0.11972120991512014
1103	10-11	0.07815300502883105
1103	12-13	0.04659379480184356
1103	14-15	0.07011468685422528
1103	16-17	0.027479668386092726
1103	18-19	0.03778329567094829
1103	20-21	-0.022014717100731218
1103	22-23	-0.05271278709966509
1103	24-25	0.020559019066233475
1103	26-27	-0.07525177220057344
1103	28-29	-0.2867320010198853
1103	30-31	-0.1736960645706631
1103	32-33	-0.23945473940869277
1103	34-35	-0.35247974093257284
1103	36-37	-0.3781258944454038
1103	38-39	-0.3485998833065054
1103	40-41	-0.21487038290635496
1103	42-43	-0.19743469838921612
1103	44-45	-0.23131268977169128
1103	46-47	-0.1830414740432431
1103	48-49	-0.22572624789829376
1103	50-51	-0.3060746349318464
1103	52-53	-0.21247201925346815
1103	54-55	-0.0948007011621641
1103	56-57	0.09526422695059011
1103	58-59	0.05449095965671091
1103	60-61	-0.02847310442422213
1103	62-63	-0.030414893748996974
1103	64-65	-0.0591358300773166
1103	66-67	-0.03983429337993982
1103	68-69	-0.07915859950448834
1103	70-71	-0.1542541043130612
1103	72-73	-0.11697620485093552
1103	74-75	-0.0912214140416836
1103	76-77	-0.13672671157066674
1103	78-79	-0.15020979130259704
1103	80-81	-0.16247105522853644
1103	82-83	-0.14208462077125716
1103	84-85	-0.2040810238604216
1103	86-87	-0.8928209385776498
1103	88-89	-0.3974056348900561
1103	90-91	-0.5601551172968939
1103	92-93	-0.28093026715754377
1103	94-95	-0.6911559709974533
1103	96-97	-0.44089035615310124
1103	98-99	-0.40612199128845106
1103	100	-0.39179031076498205
1104	1	0.09248412467631795
1104	2	-0.02185693867046723
1104	3	0.054008717570496856
1104	4	0.10274109593329683
1104	5	-0.020273721661126842
1104	6	0.12694118398487575
1104	7	0.01796867444679151
1104	8	0.07233235315757014
1104	9	0.08692808745871616
1104	10-11	-0.06007623860556777
1104	12-13	0.03249225016936208
1104	14-15	-0.07897295960437845
1104	16-17	0.041134125770888375
1104	18-19	0.030957357104398397
1104	20-21	-0.007427850581848361
1104	22-23	-0.039445132900304714
1104	24-25	-0.029123023554838312
1104	26-27	-0.006111347866458061
1104	28-29	0.02598793611580419
1104	30-31	-0.044843690152688964
1104	32-33	0.006420962830262056
1104	34-35	0.03626215179932757
1104	36-37	0.03632070020878331
1104	38-39	0.07525172876438546
1104	40-41	0.12752925116703295
1104	42-43	-0.12338475879056432
1104	44-45	0.03006399179006536
1104	46-47	0.08044327315074185
1104	48-49	-0.014195572224210196
1104	50-51	-0.06470850894520197
1104	52-53	-0.02473923950140744
1104	54-55	0.15050241976954482
1104	56-57	0.08855111575346797
1104	58-59	0.0340197118704495
1104	60-61	-0.0077382018287295296
1104	62-63	0.15405199106621836
1104	64-65	0.050228841585649775
1104	66-67	0.1389159265278721
1104	68-69	0.05946941030045849
1104	70-71	0.10898692638021146
1104	72-73	0.20776324837027715
1104	74-75	0.09873225871202607
1104	76-77	0.11460903781652831
1104	78-79	0.09894048490161467
1104	80-81	0.08706836984447364
1104	82-83	0.16471621898338284
1104	84-85	0.025704228828320907
1104	86-87	-0.02715142190570674
1104	88-89	0.08790381938671032
1104	90-91	0.022188220375952028
1104	92-93	0.019971648092916183
1104	94-95	0.030676242272075882
1104	96-97	-0.014578122241555747
1104	98-99	-0.06684610374050948
1104	100	-0.09575005883858978
1105	1	0.06392419555690054
1105	2	0.14738024799317984
1105	3	0.11929760859618099
1105	4	0.14059297474948096
1105	5	0.09629598787959281
1105	6	0.17206748008529615
1105	7	0.08155057059391879
1105	8	-0.05085104576777155
1105	9	0.05776942805410812
1105	10-11	0.04199363005015044
1105	12-13	0.12964768606432386
1105	14-15	0.047184035388653456
1105	16-17	0.06605258791869062
1105	18-19	0.07300932246082681
1105	20-21	0.06665549820510108
1105	22-23	-0.0068073725068913404
1105	24-25	0.15108169678051553
1105	26-27	0.11610229664545102
1105	28-29	0.046747658729138664
1105	30-31	8.617387595393211E-4
1105	32-33	-0.05332344582284776
1105	34-35	0.0016241670423156052
1105	36-37	-0.004196022892195117
1105	38-39	0.06283599675525409
1105	40-41	0.061405910713588696
1105	42-43	0.03055092211861421
1105	44-45	-0.09522412083130405
1105	46-47	0.017935803903434078
1105	48-49	0.002216670702047452
1105	50-51	-0.1623809426720868
1105	52-53	-0.008237709021152284
1105	54-55	0.10190966030334181
1105	56-57	0.15074942112148193
1105	58-59	0.15916011082554604
1105	60-61	0.1263640576042775
1105	62-63	0.1313322463828399
1105	64-65	0.07250164482564969
1105	66-67	0.1822618745899831
1105	68-69	0.13511620705222782
1105	70-71	0.10730717565274261
1105	72-73	0.0477637978321539
1105	74-75	0.04989961335074611
1105	76-77	0.10980449742993414
1105	78-79	0.11888471452173377
1105	80-81	0.12797031150536142
1105	82-83	0.07679426088443009
1105	84-85	0.0886470550312417
1105	86-87	0.07418976873238137
1105	88-89	0.05597816157782631
1105	90-91	-0.002030135771494912
1105	92-93	0.06105225643878498
1105	94-95	0.07779996963825297
1105	96-97	0.04763497280847773
1105	98-99	0.14733097408389284
1105	100	0.09611186139328254
1106	1	6.531656962351917E-4
1106	2	-0.002377834754888397
1106	3	0.03801510685514842
1106	4	0.07722408159428795
1106	5	0.022474472131996492
1106	6	0.0815854225867696
1106	7	0.0691479737473486
1106	8	-1.1150427992094762
1106	9	0.022348829958069416
1106	10-11	0.0350369057357085
1106	12-13	-0.006182938579094355
1106	14-15	-0.03261486551633652
1106	16-17	-0.077689621131114
1106	18-19	0.014479286347800269
1106	20-21	-0.06094016766739685
1106	22-23	0.007223810449879409
1106	24-25	0.02828919777341099
1106	26-27	-0.02327679491375534
1106	28-29	-0.08801816630673187
1106	30-31	-0.03932221351396947
1106	32-33	-0.052065188331503975
1106	34-35	-0.015944942619746882
1106	36-37	0.08715044662721994
1106	38-39	-0.01716102303112166
1106	40-41	0.059425286869078775
1106	42-43	0.02733976842195318
1106	44-45	-0.07258845380061274
1106	46-47	0.040567798632970664
1106	48-49	-0.044617509982408876
1106	50-51	0.0369030733635185
1106	52-53	0.06511423770011504
1106	54-55	0.12951146139050707
1106	56-57	0.07498864788316695
1106	58-59	0.06367365142085646
1106	60-61	-0.005976495008191307
1106	62-63	0.07619371279448472
1106	64-65	0.02936181675113403
1106	66-67	0.022920330544486944
1106	68-69	0.03932512320520232
1106	70-71	0.11073482739183049
1106	72-73	0.06473983085884782
1106	74-75	0.02331501319857665
1106	76-77	-0.014932096618451851
1106	78-79	0.03477986396805832
1106	80-81	0.0442080266207725
1106	82-83	0.06605716736778078
1106	84-85	0.06278084879110324
1106	86-87	-0.05527900907194905
1106	88-89	-0.010499669949531665
1106	90-91	-0.03348917721085698
1106	92-93	0.013325362297187837
1106	94-95	-0.01065806730315444
1106	96-97	-0.030066382067609254
1106	98-99	0.017671510276940694
1106	100	-0.04720255105574722
1107	1	-0.005205406600982343
1107	2	-0.04950717310461883
1107	3	-0.05494710396832403
1107	4	0.16610335130634724
1107	5	0.08365614466772087
1107	6	0.060347468567549356
1107	7	0.1401496401407769
1107	8	0.07134492669126757
1107	9	0.17423493410092306
1107	10-11	0.16906462756310958
1107	12-13	0.15453908967661079
1107	14-15	0.1915012508184759
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2316	96-97	0.7681537926840392
2316	98-99	0.7761759890016613
2316	100	0.40289877565308885
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	13398.0
1	1251.0
2	731.0
3	575.0
4	506.0
5	539.0
6	530.0
7	1122.0
8	2562.0
9	4079.0
10	6267.0
11	9833.0
12	14247.0
13	18401.0
14	21885.0
15	25775.0
16	30738.0
17	37199.0
18	46002.0
19	55220.0
20	65365.0
21	76154.0
22	87312.0
23	95438.0
24	98531.0
25	102790.0
26	110189.0
27	123318.0
28	142158.0
29	167626.0
30	204074.0
31	251381.0
32	320030.0
33	427474.0
34	618758.0
35	992005.0
36	1110922.0
37	1069196.0
38	965889.0
39	201935.0
40	212.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.56305718677911	16.912714916089293	11.924592602415586	23.599635294716006
2	16.950082281952824	19.734204479400507	39.64699585390493	23.668717384741736
3	19.269624709930383	26.338721293110346	29.211810834600303	25.179843162358967
4	13.142288866137244	15.716492101805207	36.03015006350056	35.111068968556985
5	13.533709744274217	36.12896875885214	33.27719977146479	17.06012172540886
6	30.90883425836854	34.397580348368095	19.51615231563599	15.17743307762738
7	28.694874660365205	31.61337457292391	21.732268077954984	17.9594826887559
8	27.65152267453384	33.162539603240475	20.481238437599927	18.704699284625754
9	28.6482453332665	15.487541624235353	19.8616273707934	36.00258567170474
10-11	25.885424614775637	24.975066955702946	28.066611178453694	21.072897251067726
12-13	26.147917802412824	23.027560477233315	27.961535026744738	22.86298669360912
14-15	23.81817463449495	24.35402861250365	26.03939847634244	25.78839827665896
16-17	23.16612278182073	26.758641332254225	26.706747161712713	23.368488724212337
18-19	22.93679547236323	25.909563222521104	28.657372617564665	22.496268687550998
20-21	23.837733502439796	25.427728346207694	28.387306938694284	22.34723121265822
22-23	23.57258811016472	25.08679690773043	28.33934280708673	23.001272175018123
24-25	23.156023276463817	25.180909034873046	28.36603955266275	23.297028136000385
26-27	22.919741104205357	25.23803526532288	28.464943582280313	23.377280048191455
28-29	22.96603968234927	25.674621263618647	28.431086372954372	22.92825268107771
30-31	23.890270060283633	25.29930254361351	28.399315270676645	22.411112125426207
32-33	23.48526105930363	25.407422424067462	28.344241293025064	22.763075223603842
34-35	23.616485699367825	25.66605063424065	28.106970290516948	22.610493375874576
36-37	23.56859639543418	25.688805364347527	27.986149325691557	22.756448914526736
38-39	23.54670336517131	25.92017808690386	27.737337809761915	22.795780738162915
40-41	23.489189374544164	25.49496367021746	28.053980780295706	22.96186617494267
42-43	23.22967239732283	25.803659636541987	27.700817032500375	23.26585093363481
44-45	22.945431062662603	25.903164666015737	27.895331391027966	23.256072880293694
46-47	23.01912995225008	25.579882799407827	27.63751639650224	23.763470851839852
48-49	23.135696188124232	25.759960507356254	27.64180523977234	23.46253806474717
50-51	23.018188130255147	26.081487594549724	27.615139683547657	23.28518459164747
52-53	23.106081710243917	26.23768037745476	27.169001322499202	23.487236589802123
54-55	22.879831984862108	25.960365010354312	27.609090200161162	23.550712804622414
56-57	23.08761456843941	26.102272703794178	27.18109669653266	23.629016031233757
58-59	23.018879417944817	26.060316086760178	27.51796662438598	23.402837870909025
60-61	23.51124918647724	26.097363656517516	27.501250561144957	22.890136595860284
62-63	23.33726584084009	26.063373358261433	27.490906909548997	23.108453891349484
64-65	23.208545065503262	25.757768733652508	27.394595452011316	23.63909074883291
66-67	23.040499450656533	25.89515258044796	27.25222156844292	23.81212640045259
68-69	22.814284030761716	25.894461632640436	27.405565349450068	23.88568898714778
70-71	22.773023459108067	25.7266960641655	27.576689083915145	23.92359139281129
72-73	22.748703619384397	26.18465148559121	27.039639613089733	24.027005281934656
74-75	22.83248284298204	26.194151781696736	27.055725727694206	23.917639647627016
76-77	22.71773620816147	25.81968584547499	27.1892474313846	24.27333051497894
78-79	22.58636932255357	25.870725594496808	27.299236364719853	24.243668718229774
80-81	22.751854876182968	26.099529492871216	26.79851876496744	24.350096865978383
82-83	22.989535007732094	25.983935903588762	26.95276755719085	24.073761531488294
84-85	22.92687798073266	26.225007709524068	26.601324478235544	24.246789831507733
86-87	22.62461044394509	26.16147579661476	26.951543102915586	24.262370656524563
88-89	22.524263392029805	25.760980023182782	27.18655846612858	24.528198118658835
90-91	23.11409534123294	25.70799270188065	27.00749443078782	24.17041752609859
92-93	22.898196500486375	25.742654297670057	27.00134916574852	24.35780003609505
94-95	22.784224448393374	25.499885278460038	26.969774173881593	24.746116099264995
96-97	22.686003354246267	25.453976434953486	27.090264686825915	24.76975552397433
98-99	22.693372574892756	25.5001842530646	26.81579578961919	24.99064738242345
100	22.705237810796685	25.503501295986176	26.92225949589871	24.86900139731843
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6478.0
1	7110.5
2	8941.0
3	7974.5
4	4344.0
5	3097.5
6	4038.0
7	6634.5
8	10052.0
9	11687.5
10	10426.5
11	8841.0
12	9010.0
13	9641.0
14	10203.5
15	10768.0
16	11357.5
17	12070.0
18	12787.5
19	13312.0
20	14040.0
21	15139.5
22	16643.0
23	18776.0
24	21023.5
25	24266.0
26	29610.5
27	35906.0
28	41660.5
29	49266.0
30	59615.0
31	68779.0
32	80051.5
33	93159.5
34	106107.0
35	119170.5
36	135230.0
37	152343.0
38	163592.5
39	173568.5
40	181184.5
41	188211.5
42	198047.0
43	209626.5
44	222240.5
45	236340.0
46	251531.0
47	267301.5
48	286962.5
49	306758.0
50	323561.5
51	327697.0
52	318552.0
53	308799.5
54	301205.5
55	286861.0
56	265148.0
57	245460.0
58	225394.5
59	198131.0
60	165388.0
61	135215.0
62	110577.0
63	87374.0
64	65952.0
65	49867.0
66	37514.0
67	29239.0
68	22799.0
69	17163.0
70	13484.5
71	10707.0
72	8468.5
73	6712.0
74	5254.5
75	4006.0
76	2967.5
77	1983.5
78	1242.0
79	852.0
80	627.0
81	432.0
82	303.5
83	241.0
84	178.0
85	125.5
86	99.5
87	75.0
88	58.5
89	47.0
90	39.5
91	27.5
92	22.5
93	19.0
94	15.0
95	16.0
96	12.5
97	6.5
98	5.0
99	4.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14484917272442882
2	0.07163353305545869
3	0.31132933250921974
4	0.30921542535335156
5	0.31148887267192676
6	0.2576573627718614
7	0.2584683585989555
8	0.25779031290745064
9	0.2695962849477712
10-11	0.24625024113830843
12-13	0.25535732542616835
14-15	0.229212681262553
16-17	0.23776802248771772
18-19	0.25120928119578545
20-21	0.25321018073640283
22-23	0.24363777097398073
24-25	0.2436909510282164
26-27	0.24212878693504336
28-29	0.253097173121152
30-31	0.2382732330029567
32-33	0.25046476043648597
34-35	0.23509572476237492
36-37	0.2432987481282283
38-39	0.24348487831805316
40-41	0.2602698329361891
42-43	0.24661585401117872
44-45	0.23234365695567855
46-47	0.23572059039964413
48-49	0.24525976262816893
50-51	0.23877179601141615
52-53	0.2546659847211045
54-55	0.26473030998520664
56-57	0.26065538832939783
58-59	0.257564297676949
60-61	0.2391174663639481
62-63	0.2664586617478662
64-65	0.2593790670277415
66-67	0.2650360952970618
68-69	0.2704870508562188
70-71	0.29097466675051387
72-73	0.3000086284637998
74-75	0.27975367530678574
76-77	0.27635015183570233
78-79	0.27372438665781573
80-81	0.25923946938537284
82-83	0.27144429183246105
84-85	0.28124271682538476
86-87	0.27401687695611193
88-89	0.28216007276095023
90-91	0.2762770292611283
92-93	0.2553041453719327
94-95	0.2771478526492375
96-97	0.2749009953577801
98-99	0.26199818469884867
100	0.286334707018451
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7521617.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.158859580907304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.12532341532855	39.60384122659212
2	15.596893492336225	16.89419929099609
3	5.219163492964734	8.479918282358241
4	2.1470295036880196	4.651226776252182
5	1.0751597605402134	2.9114713249069673
6	0.6587594580509779	2.1406596591706504
7	0.4166217377788789	1.579463073630392
8	0.29928189874319683	1.2967013063312092
9	0.21454952532158605	1.0457781853537886
>10	1.1309125882106026	11.01337903020326
>50	0.07152112607877655	2.6567281688999227
>100	0.041050929070987084	4.066537806900812
>500	0.002466499095139938	0.9078563476991723
>1k	0.0010665876143629122	1.1117448290789045
>5k	6.666172589303052E-5	0.19475887704346154
>10k+	1.3332345178606103E-4	1.4457358145827963
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29805	0.396257879123598	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23977	0.31877454010221473	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15212	0.20224374625828465	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11095	0.14750817543621272	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0636010847135663E-4
2	0.0	0.0	0.0	0.0	1.0636010847135663E-4
3	0.0	0.0	0.0	0.0	1.0636010847135663E-4
4	0.0	0.0	0.0	1.3295013558919579E-5	1.0636010847135663E-4
5	0.0	0.0	0.0	1.3295013558919579E-5	1.0636010847135663E-4
6	0.0	0.0	0.0	1.3295013558919579E-5	1.196551220302762E-4
7	0.0	0.0	0.0	3.9885040676758736E-5	1.3295013558919577E-4
8	0.0	0.0	0.0	5.3180054235678315E-5	1.4624514914811536E-4
9	0.0	0.0	0.0	6.647506779459789E-5	1.5954016270703495E-4
10-11	6.647506779459789E-6	0.0	0.0	7.312257457405768E-5	1.661876694864947E-4
12-13	1.3295013558919579E-5	0.0	0.0	7.977008135351747E-5	1.794826830454143E-4
14-15	1.3295013558919579E-5	6.647506779459789E-6	0.0	7.977008135351747E-5	2.7919528473731113E-4
16-17	1.3295013558919579E-5	1.3295013558919579E-5	0.0	1.1300761525081642E-4	3.8555539320866777E-4
18-19	1.3295013558919579E-5	1.3295013558919579E-5	0.0	1.3295013558919577E-4	4.1214542032650694E-4
20-21	1.3295013558919579E-5	1.3295013558919579E-5	0.0	2.3266273728109264E-4	4.453829542238059E-4
22-23	1.3295013558919579E-5	1.3295013558919579E-5	0.0	6.115706237103006E-4	5.318005423567831E-4
24-25	1.3295013558919579E-5	1.3295013558919579E-5	0.0	0.0015621640931730504	6.049231169308408E-4
26-27	1.3295013558919579E-5	1.3295013558919579E-5	0.0	0.0027853053405936514	6.3151314404868E-4
28-29	1.3295013558919579E-5	1.3295013558919579E-5	0.0	0.0067738094082695245	6.448081576075996E-4
30-31	1.3295013558919579E-5	1.3295013558919579E-5	0.0	0.019470547357037724	6.713981847254386E-4
32-33	1.3295013558919579E-5	1.3295013558919579E-5	0.0	0.04957710556121111	7.312257457405768E-4
34-35	1.3295013558919579E-5	1.3295013558919579E-5	0.0	0.09465384903272794	7.445207592994964E-4
36-37	1.3295013558919579E-5	1.3295013558919579E-5	0.0	0.1655628038492255	7.578157728584159E-4
38-39	1.3295013558919579E-5	1.3295013558919579E-5	0.0	0.27377091920527197	7.578157728584159E-4
40-41	1.9942520338379368E-5	1.3295013558919579E-5	0.0	0.4051522431945152	7.578157728584159E-4
42-43	2.6590027117839158E-5	1.3295013558919579E-5	0.0	0.5259174988569613	7.844057999762551E-4
44-45	2.6590027117839158E-5	1.3295013558919579E-5	0.0	0.6683336841001077	8.109958270940943E-4
46-47	2.6590027117839158E-5	1.3295013558919579E-5	0.0	0.8349002614730316	8.375858542119335E-4
48-49	2.6590027117839158E-5	1.3295013558919579E-5	0.0	1.013691603813382	8.907659084476117E-4
50-51	2.6590027117839158E-5	1.3295013558919579E-5	0.0	1.1983327521196572	9.040609220065313E-4
52-53	2.6590027117839158E-5	1.3295013558919579E-5	0.0	1.3865502590732817	9.705359898011291E-4
54-55	2.6590027117839158E-5	1.3295013558919579E-5	0.0	1.6056387875107174	0.0010303635508162672
56-57	2.6590027117839158E-5	2.6590027117839158E-5	0.0	1.847667861844069	0.001037011057595727
58-59	2.6590027117839158E-5	2.6590027117839158E-5	0.0	2.124569756742466	0.0010569535779341064
60-61	2.6590027117839158E-5	2.6590027117839158E-5	0.0	2.4072416875254348	0.0011300761525081641
62-63	2.6590027117839158E-5	2.6590027117839158E-5	0.0	2.6934235018879584	0.0011832562067438425
64-65	2.6590027117839158E-5	2.6590027117839158E-5	0.0	2.985947037718087	0.0011899037135233023
66-67	2.6590027117839158E-5	2.6590027117839158E-5	0.0	3.312140195386178	0.001229788754200061
68-69	2.6590027117839158E-5	2.6590027117839158E-5	0.0	3.695834286696597	0.0012696737948768198
70-71	2.6590027117839158E-5	2.6590027117839158E-5	0.0	4.113616792772087	0.0013095588355535785
72-73	2.6590027117839158E-5	2.6590027117839158E-5	0.0	4.485657804698112	0.001542221572834671
74-75	2.6590027117839158E-5	2.6590027117839158E-5	0.0	4.855013223885236	0.0015621640931730504
76-77	2.6590027117839158E-5	2.6590027117839158E-5	0.0	5.265430292449084	0.0015688115999525102
78-79	2.6590027117839158E-5	2.6590027117839158E-5	0.0	5.734830688667078	0.0015688115999525102
80-81	2.6590027117839158E-5	3.9885040676758736E-5	0.0	6.2031674838003585	0.0015821066135114298
82-83	2.6590027117839158E-5	4.653254745621852E-5	0.0	6.686913199648426	0.0016552291880854875
84-85	2.6590027117839158E-5	5.3180054235678315E-5	0.0	7.179626402141986	0.0016618766948649473
86-87	2.6590027117839158E-5	5.3180054235678315E-5	0.0	7.694349499582337	0.0016618766948649473
88	2.6590027117839158E-5	5.3180054235678315E-5	0.0	8.107711413649485	0.001701761735541706
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	15565	0.0	59.217754	2
GTACCTG	17745	0.0	52.38531	1
ACCTGGG	17655	0.0	51.568275	3
CCTGGGG	15010	0.0	41.82146	4
GGTATCA	18845	0.0	36.24675	1
GTATCAA	29545	0.0	33.803726	1
TATAACG	875	0.0	31.709465	2
CTGGGGG	9430	0.0	29.666098	5
GTACCCG	1515	0.0	28.257082	1
TCAACGC	35205	0.0	27.835047	4
TGGGGGG	15230	0.0	27.645401	6
TATCAAC	35935	0.0	27.377197	2
CAACGCA	35910	0.0	27.298943	5
GTACATG	32490	0.0	27.29356	1
ATCAACG	35955	0.0	27.283491	3
TAACGCA	985	0.0	27.210545	4
TATCACG	710	0.0	27.15629	2
AACGCAG	37120	0.0	26.546377	6
ATAACGC	1005	0.0	26.203959	3
TACATGG	33690	0.0	26.172503	2
>>END_MODULE