FastQCFastQC Report
Mon 6 Jun 2016
ERR523206_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523206_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7521617
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259130.344513686352283No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225110.2992840502248386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143200.19038459416372835No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94080.12507948756231538No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG154350.061.169152
GTACCTG177000.053.890871
ACCTGGG175400.053.2348063
CCTGGGG149450.043.8008544
GGTATCA184700.035.9142271
TATAACG9250.035.5643962
CTGGGGG90750.034.1762735
GTATCAA285900.032.4922071
TAACGCA10550.031.6252024
ATAACGC11200.030.6289583
GTACCCG16350.030.1910341
TGGGGGG137950.028.6144226
TCAACGC347950.026.3087064
CAACGCA354000.025.8989075
TATCAAC355750.025.7733882
ATCAACG355300.025.7644633
GTACATG323750.025.6150511
AACGCAG367850.025.1026296
CATGGGG214650.024.826164
TACATGG332200.024.6296542
Produced by FastQC (version 0.11.5)