##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR523206_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7521617 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2561587488435 33.0 31.0 34.0 30.0 34.0 2 32.383799520767944 34.0 31.0 34.0 30.0 34.0 3 32.57728105539008 34.0 31.0 34.0 30.0 35.0 4 35.6714548214832 37.0 35.0 37.0 33.0 37.0 5 35.60153700992752 36.0 35.0 37.0 33.0 37.0 6 35.71918259065836 37.0 35.0 37.0 33.0 37.0 7 35.562392368555855 37.0 35.0 37.0 33.0 37.0 8 35.54687855018409 36.0 35.0 37.0 33.0 37.0 9 36.8447444213126 38.0 37.0 39.0 33.0 39.0 10-11 36.63903685869674 37.5 35.0 39.0 33.0 39.0 12-13 36.64058007739559 37.5 35.0 39.0 33.0 39.0 14-15 37.822537028939394 38.0 37.0 41.0 33.0 41.0 16-17 37.632119396666965 38.0 36.5 41.0 33.0 41.0 18-19 37.53253908035998 38.0 36.0 41.0 32.0 41.0 20-21 37.54663465316035 38.0 36.5 41.0 33.0 41.0 22-23 37.40176121703618 38.0 36.0 41.0 33.0 41.0 24-25 37.32900611663689 38.0 36.5 41.0 32.0 41.0 26-27 37.01667507133107 38.0 36.0 40.0 32.5 41.0 28-29 36.978111488527 38.0 36.0 40.0 32.0 41.0 30-31 36.73023952163477 38.0 35.5 40.0 32.0 41.0 32-33 36.67541234816928 37.5 36.0 40.0 30.5 41.0 34-35 36.44811215460718 38.0 35.0 40.0 30.5 41.0 36-37 36.22295478485544 37.0 35.5 40.0 30.5 41.0 38-39 36.177555504354984 37.0 35.0 40.0 30.0 41.0 40-41 36.37185308690937 37.5 35.5 40.0 30.5 41.0 42-43 36.416349037713566 37.5 35.5 40.0 30.5 41.0 44-45 36.2321425698756 37.0 35.0 40.0 30.5 41.0 46-47 36.12247758693377 37.0 35.5 40.0 30.0 41.0 48-49 35.90491791858054 38.0 35.0 40.0 29.5 41.0 50-51 35.739583988389725 38.0 34.5 40.0 28.5 41.0 52-53 35.75017765461868 37.5 35.0 40.0 28.5 41.0 54-55 35.548600121489834 37.5 34.5 40.0 28.0 41.0 56-57 35.300838902060555 37.0 35.0 40.0 27.5 41.0 58-59 35.11378670304536 37.0 34.0 40.0 27.5 41.0 60-61 34.92552412599578 37.0 34.0 39.0 27.0 41.0 62-63 34.670276351481334 36.5 34.0 39.0 26.0 41.0 64-65 34.48152026352844 37.0 33.5 38.5 26.0 40.5 66-67 33.97587500134612 35.5 33.5 38.0 26.0 40.0 68-69 33.83171876472838 36.5 33.0 37.0 25.0 40.0 70-71 33.41795301462438 35.0 33.0 37.0 25.5 39.5 72-73 33.187991691148326 35.0 33.0 37.0 25.0 39.0 74-75 33.00256992612094 35.0 32.5 37.0 24.0 39.0 76-77 31.394134200132765 34.0 30.0 36.0 22.5 37.0 78-79 32.50387077672261 35.0 32.0 36.5 23.5 37.0 80-81 32.62306196925475 35.0 32.0 36.0 24.0 37.5 82-83 32.519770881713335 35.0 32.0 37.0 24.5 37.0 84-85 32.186976417437904 35.0 32.0 36.0 23.5 36.5 86-87 32.0634910020013 35.0 32.0 36.0 24.0 36.5 88-89 32.02947637456148 35.0 32.0 36.0 23.0 37.0 90-91 31.789732912484112 34.5 32.0 35.0 23.0 36.5 92-93 31.576683045680205 35.0 31.5 35.0 21.5 36.5 94-95 31.712408781250097 35.0 31.0 35.0 21.0 37.0 96-97 31.434191610660314 35.0 31.0 35.0 21.0 37.0 98-99 31.283001514169094 34.0 31.0 35.0 20.0 36.0 100 30.035706284965055 34.0 29.0 35.0 18.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.06228355863874668 1101 2 -0.10627679151667024 1101 3 -0.11515919180506273 1101 4 -0.09795255240560863 1101 5 -0.17193946545643968 1101 6 -0.11007930075853523 1101 7 -0.06303131746263091 1101 8 -0.13096958189317576 1101 9 -0.1285598532835337 1101 10-11 -0.1490790785602698 1101 12-13 -0.136684039729289 1101 14-15 -0.2751303952674249 1101 16-17 -0.26976676887963436 1101 18-19 -0.3827946617935325 1101 20-21 -0.33750394347516277 1101 22-23 -0.7931964988391726 1101 24-25 -0.31474384924249676 1101 26-27 -0.2760155306149059 1101 28-29 -0.35642834305007653 1101 30-31 -0.33094178046912504 1101 32-33 -0.27472929391434775 1101 34-35 -0.3504114581734612 1101 36-37 -0.3609943199492065 1101 38-39 -0.3737307021583689 1101 40-41 -0.32445215700506225 1101 42-43 -0.30426421476933996 1101 44-45 -0.3837678523144987 1101 46-47 -0.2634680196449608 1101 48-49 -0.39574235122794477 1101 50-51 -0.34265683799185354 1101 52-53 -0.443689826719897 1101 54-55 -0.4323216532617593 1101 56-57 -0.51945829209631 1101 58-59 -0.5003180472442565 1101 60-61 -0.6643685078630597 1101 62-63 -0.5042655044919471 1101 64-65 -0.4647412755732816 1101 66-67 -0.4081636180338464 1101 68-69 -0.400031837919542 1101 70-71 -0.5275644485318054 1101 72-73 -0.46736452545265905 1101 74-75 -0.4024927176591646 1101 76-77 -0.5619511856650092 1101 78-79 -0.5062689808864036 1101 80-81 -0.43637283196927257 1101 82-83 -0.36863479953483136 1101 84-85 -0.41278259780105486 1101 86-87 -0.5927944949390422 1101 88-89 -0.5041024291814331 1101 90-91 -0.5686399658020349 1101 92-93 -0.46477472855251634 1101 94-95 -0.6081020527780545 1101 96-97 -0.6244466079826125 1101 98-99 -0.5825766959326444 1101 100 -0.6317523200377018 1102 1 -0.03837726628395899 1102 2 -0.11444118049277563 1102 3 -0.16790549922823317 1102 4 -0.14286618740626977 1102 5 -0.138873263541619 1102 6 -0.19636117622302152 1102 7 -0.14213987345740264 1102 8 -0.17499467054009443 1102 9 -0.13254668343531506 1102 10-11 -0.26353590392417203 1102 12-13 -0.13660707318017273 1102 14-15 -0.2538522787379236 1102 16-17 -0.29527290832506736 1102 18-19 -0.30414135830022815 1102 20-21 -0.33168447487744146 1102 22-23 -0.27568514376945075 1102 24-25 -0.19944890452413944 1102 26-27 -0.23738184672954077 1102 28-29 -0.27451900215228164 1102 30-31 -0.29914259632196405 1102 32-33 -0.3416533033381697 1102 34-35 -0.33575074007519135 1102 36-37 -0.4646716370058357 1102 38-39 -0.48837304472486665 1102 40-41 -0.4850706624192753 1102 42-43 -0.4941982401562015 1102 44-45 -0.32887540221738476 1102 46-47 -0.31390646191024985 1102 48-49 -0.4255438361370807 1102 50-51 -0.3011256695439357 1102 52-53 -0.4612178918765508 1102 54-55 -0.396877667179389 1102 56-57 -0.4346122971536275 1102 58-59 -0.42169447323967546 1102 60-61 -0.4863093267812104 1102 62-63 -0.5435520872676634 1102 64-65 -0.5516076517032786 1102 66-67 -0.6106072670212797 1102 68-69 -0.5154615761027088 1102 70-71 -0.44519596043969045 1102 72-73 -0.4609931788785602 1102 74-75 -0.5493073374013875 1102 76-77 -0.5522998585984951 1102 78-79 -0.5021960965688663 1102 80-81 -0.5170370395639665 1102 82-83 -0.5013825491071131 1102 84-85 -0.5607169231905544 1102 86-87 -0.4629224011032065 1102 88-89 -0.48767869490838933 1102 90-91 -0.5013758755080673 1102 92-93 -0.510424275039643 1102 94-95 -0.6490028930079603 1102 96-97 -0.617832900236504 1102 98-99 -0.7452021922092449 1102 100 -0.7411788713977217 1103 1 -0.10519918302091469 1103 2 -0.11541100001316806 1103 3 -0.201912786932418 1103 4 -0.026453305635030233 1103 5 -0.10525748977698157 1103 6 -0.15326851959975585 1103 7 -0.11143152871957795 1103 8 -0.1355923963444141 1103 9 -0.08149011528485062 1103 10-11 -0.217373995491549 1103 12-13 -0.17878850610592423 1103 14-15 -0.15310542870561505 1103 16-17 -0.2314690302386424 1103 18-19 -0.30119742558393625 1103 20-21 -0.2654693051320365 1103 22-23 -0.2847112093215429 1103 24-25 -0.23352179832362907 1103 26-27 -0.2562289781037208 1103 28-29 -0.29289611012165295 1103 30-31 -0.37989681039874057 1103 32-33 -0.2956689046854848 1103 34-35 -0.3359232225681268 1103 36-37 -0.3857271810793179 1103 38-39 -0.26383415865995374 1103 40-41 -0.316928058768724 1103 42-43 -0.33325144374829563 1103 44-45 -0.19812297562042858 1103 46-47 -0.2513682684937635 1103 48-49 -0.3394291655826507 1103 50-51 -0.3562796162435262 1103 52-53 -0.2954922309279553 1103 54-55 -1.0325085358387227 1103 56-57 -0.3333792712295818 1103 58-59 -0.2684063296305723 1103 60-61 -0.2826225100034776 1103 62-63 -0.2883544124809987 1103 64-65 -0.2710039117741445 1103 66-67 -0.3203739078067329 1103 68-69 -0.2555148932742455 1103 70-71 -0.31519493636271534 1103 72-73 -0.4392805870142027 1103 74-75 -0.4806894223848772 1103 76-77 -0.3813266596240581 1103 78-79 -0.3534609805968074 1103 80-81 -1.0877223146403558 1103 82-83 -0.1748177874098289 1103 84-85 -0.15678185153308988 1103 86-87 -0.29450443246230407 1103 88-89 -0.27658678679230064 1103 90-91 -0.2997431057894673 1103 92-93 -0.2802657106838531 1103 94-95 -0.29918901434432854 1103 96-97 -0.395633331838404 1103 98-99 -0.31903486547841453 1103 100 -0.2883464792842325 1104 1 -0.08285091941301914 1104 2 -0.1919065490790004 1104 3 -0.13950869606064487 1104 4 -0.0362920399042892 1104 5 -0.08191840951942453 1104 6 -0.2417561402558448 1104 7 -0.15397096552100464 1104 8 -0.11680253438787958 1104 9 -0.17479717696459574 1104 10-11 -0.1609291131862136 1104 12-13 -0.15596500705006378 1104 14-15 -0.32164160500717287 1104 16-17 -0.33647636049912677 1104 18-19 -0.2858268662557464 1104 20-21 -0.32640241797113134 1104 22-23 -0.24576090262344508 1104 24-25 -0.2056400676784449 1104 26-27 -0.2760985581091191 1104 28-29 -0.4293231652334768 1104 30-31 -0.3820665675032302 1104 32-33 -0.23876403387399137 1104 34-35 -0.37850019030324944 1104 36-37 -0.5581564880471674 1104 38-39 -0.30628961999723003 1104 40-41 -0.244516109857706 1104 42-43 -0.18094716170332958 1104 44-45 -0.30977070089399206 1104 46-47 -0.19101477401198252 1104 48-49 -0.23883734967187564 1104 50-51 -0.17240994241079477 1104 52-53 -0.2593208553019508 1104 54-55 -0.25582275185270476 1104 56-57 -0.18999140075820975 1104 58-59 -0.27690051820005834 1104 60-61 -0.39236061445675574 1104 62-63 -0.37878214784100095 1104 64-65 -0.5435256386662317 1104 66-67 -0.3886105403436986 1104 68-69 -0.30581525802800513 1104 70-71 -0.22959109882474138 1104 72-73 -0.28785523252280854 1104 74-75 -0.42883222758760553 1104 76-77 -0.28626971816058955 1104 78-79 -0.29238501564130814 1104 80-81 -0.3661851883370346 1104 82-83 -0.3603918347691746 1104 84-85 -1.0123714976867575 1104 86-87 -0.3709791049462652 1104 88-89 -0.39427370843474563 1104 90-91 -0.3710720526949238 1104 92-93 -0.24446542216480438 1104 94-95 -0.25585532915849285 1104 96-97 -0.23512549139178773 1104 98-99 -0.38672378095171567 1104 100 -1.5342697988953162 1105 1 0.011882625921018075 1105 2 -0.11135791140102924 1105 3 -0.15945617666349676 1105 4 -0.03347842881797902 1105 5 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-0.2319174324531943 1105 66-67 -0.2528832474278673 1105 68-69 -0.10548603879043128 1105 70-71 -0.16691572318742232 1105 72-73 -0.19166300328731012 1105 74-75 -0.21436562914579582 1105 76-77 -0.23141877114016296 1105 78-79 -0.22399789228025924 1105 80-81 -0.13789651022782579 1105 82-83 -0.10519962613850709 1105 84-85 -0.053305124982195196 1105 86-87 -0.07023105839768817 1105 88-89 -0.24552476443177795 1105 90-91 -0.21256054490286758 1105 92-93 -0.1320990959355548 1105 94-95 -0.26020290042254857 1105 96-97 -0.22878460982600402 1105 98-99 -0.17762611181475663 1105 100 -0.12044085095454093 1106 1 -0.05811706599410371 1106 2 -0.10864238594461284 1106 3 -0.0827017811352988 1106 4 -0.0518884513046487 1106 5 -0.10473749923760067 1106 6 -0.11847871771658447 1106 7 -0.09646050074516666 1106 8 -0.18115902295870256 1106 9 -0.12754399108749936 1106 10-11 -0.12658906682383275 1106 12-13 -0.18783870393333046 1106 14-15 -0.20992177550345303 1106 16-17 -0.1342275071507899 1106 18-19 -0.18025724231377893 1106 20-21 -0.19704470226788118 1106 22-23 -0.07648726028951813 1106 24-25 -0.0682227101521633 1106 26-27 -0.056148508545717846 1106 28-29 -0.07207057584746934 1106 30-31 -0.1421778062603991 1106 32-33 -0.036398231846192175 1106 34-35 -0.06777977022782267 1106 36-37 -0.1335211330643986 1106 38-39 -0.17769478451721454 1106 40-41 -0.17047229644922623 1106 42-43 -0.18482433553767663 1106 44-45 -0.14341339909181983 1106 46-47 -0.31367623526942623 1106 48-49 -0.24196742525321469 1106 50-51 -0.20851419083886213 1106 52-53 -0.3401234226724341 1106 54-55 -0.30993367278485096 1106 56-57 -0.19680468584407151 1106 58-59 -0.27143921886033695 1106 60-61 -0.22916411435680573 1106 62-63 -0.2703676256963874 1106 64-65 -0.23191231241793986 1106 66-67 -0.16076459714332003 1106 68-69 -0.1293863545288758 1106 70-71 -0.22008026249415735 1106 72-73 -0.2558050835594017 1106 74-75 -0.2351453981562841 1106 76-77 -0.22847046063262155 1106 78-79 -0.12821051604496603 1106 80-81 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-0.4799705376083345 2316 86-87 0.15972722236131887 2316 88-89 0.2140125415605567 2316 90-91 0.30317704582250116 2316 92-93 0.45709531064440867 2316 94-95 0.37977145434081905 2316 96-97 0.42107848310576657 2316 98-99 0.5260818766473143 2316 100 0.31379539425387293 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 1.0 9 2.0 10 4.0 11 9.0 12 46.0 13 255.0 14 980.0 15 2628.0 16 5653.0 17 10802.0 18 18042.0 19 26064.0 20 34674.0 21 45073.0 22 57109.0 23 66817.0 24 73383.0 25 80646.0 26 93021.0 27 109244.0 28 130806.0 29 158029.0 30 194611.0 31 240196.0 32 300152.0 33 388820.0 34 557298.0 35 976286.0 36 1555373.0 37 1124655.0 38 1060777.0 39 210030.0 40 130.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.27733436050551 17.48239247411941 12.325574033541287 23.91469913183379 2 17.052491645230713 20.03276489611419 39.01316260094931 23.901580857705788 3 19.238429715312545 26.147728606761017 29.391751800178074 25.22208987774836 4 13.312059493716829 16.012481917081217 35.7840438491752 34.891414740026754 5 13.68167508444703 36.24839943846897 33.129892008047776 16.940033469036223 6 31.212331071895843 34.499456699270915 19.176794032453394 15.111418196379848 7 28.89084886933222 31.627853425666313 21.430484960880086 18.050812744121377 8 27.773281106953906 33.48167029044564 20.146221276444127 18.59882732615633 9 28.461991616962152 15.529107637360424 19.764712827042377 36.24418791863505 10-11 25.944815187001357 25.0455138200678 27.912670102231345 21.097000890699494 12-13 26.064375450203777 23.030663447136337 27.909669147257883 22.995291955402006 14-15 23.75278798891692 24.384840733266017 25.911203999013914 25.951167278803144 16-17 23.22139818424468 26.83481402241947 26.639101678947462 23.304686114388385 18-19 22.939673976845125 25.9855432604682 28.50459735165848 22.57018541102819 20-21 23.75242403622872 25.51617712635142 28.26688821686393 22.464510620555934 22-23 23.528942916659354 25.186111558713055 28.250101973472542 23.034843551155053 24-25 23.119531393810643 25.26410552135151 28.256546904527664 23.359816180310183 26-27 22.963523611921225 25.31904780679129 28.324040037575198 23.393388543712287 28-29 22.989298664661685 25.765731433654228 28.333018753082783 22.911951148601307 30-31 23.880871574118704 25.336473482141376 28.322349460667805 22.460305483072116 32-33 23.540538531771837 25.45302342921807 28.19970379250105 22.806734246509045 34-35 23.625010985977703 25.70669586864746 28.034466651890167 22.63382649348467 36-37 23.565769132202384 25.755603870498962 27.908383610309585 22.77024338698907 38-39 23.53621165555569 25.969230511501678 27.68500107261836 22.809556760324277 40-41 23.517761178491696 25.558417684821066 27.983686660930886 22.940134475756352 42-43 23.305481482505762 25.84998159844619 27.625465623131923 23.219071295916134 44-45 22.954474094617783 25.9522238228321 27.832522712959946 23.260779369590175 46-47 23.002363563229473 25.638816955158784 27.53940767975214 23.8194118018596 48-49 23.079902726560466 25.808650623570195 27.59816037883877 23.513286271030566 50-51 23.077937767946004 26.152979370405806 27.44215863527044 23.326924226377752 52-53 23.0811969013939 26.2372242399398 27.04564905312133 23.635929805544976 54-55 22.922505071584528 25.967935407065625 27.47688636486318 23.632673156486668 56-57 23.117879953473626 26.15177338090208 26.980477044903857 23.749869620720442 58-59 23.010502130409375 26.1181474712649 27.37556434740465 23.495786050921073 60-61 23.532448186877243 26.162819182178072 27.320318564443102 22.98441406650159 62-63 23.334351732861304 26.169171849735285 27.27485991688477 23.22161650051864 64-65 23.220317868707653 25.84987281954521 27.167086613000752 23.762722698746384 66-67 23.069343308063022 25.981153727750318 27.039500751209143 23.910002212977513 68-69 22.790198059910818 26.02058228733651 27.190936305816322 23.998283346936354 70-71 22.785395276209243 25.862430338195658 27.350762453371836 24.001411932223267 72-73 22.736049823812333 26.284778591598208 26.774056927159744 24.205114657429714 74-75 22.83421926285266 26.354948976535848 26.663250986727977 24.14758077388352 76-77 22.649395941208887 25.936874476895653 26.980563619792306 24.43316596210315 78-79 22.52005806669959 25.97291536938745 27.049942693137357 24.4570838707756 80-81 22.610652447732626 26.286153477165936 26.54084615349844 24.562347921603 82-83 22.837775260860564 26.162895744718472 26.679910396092723 24.319418598328244 84-85 22.778109083120608 26.385622091166972 26.30712144878323 24.529147376929185 86-87 22.458898655882777 26.312843918261443 26.641550969734993 24.586706456120787 88-89 22.28761680518985 25.952971473508647 26.892757596284305 24.866654125017202 90-91 22.90396239622367 25.86738034603079 26.738453397787833 24.490203859957706 92-93 22.620432601782937 26.044419508793524 26.616313787690288 24.718834101733243 94-95 22.374229312838445 25.762178351180008 26.655503727889307 25.20808860809224 96-97 22.234450165984644 25.727865490284557 26.84769179040452 25.189992553326277 98-99 22.253734503344212 25.717453740619078 26.60302536068706 25.425786395349647 100 22.04153393243387 25.880792261336637 26.78760621563817 25.29006759059132 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2494.0 1 1884.0 2 3754.5 3 5427.5 4 3470.0 5 2499.0 6 3379.0 7 5794.0 8 8752.5 9 10107.5 10 9012.0 11 7662.0 12 7858.0 13 8561.0 14 9314.5 15 9904.0 16 10528.5 17 11204.0 18 11953.5 19 12729.5 20 13437.5 21 14675.0 22 16175.0 23 18062.0 24 20491.5 25 23839.0 26 29175.0 27 35424.0 28 41379.5 29 49035.0 30 59209.5 31 68901.5 32 80189.0 33 92787.5 34 105717.0 35 118886.0 36 134851.5 37 151526.0 38 163126.0 39 173265.0 40 181008.5 41 188307.5 42 197549.5 43 209305.0 44 223027.0 45 237367.5 46 252877.5 47 269852.0 48 289889.0 49 309111.0 50 325908.5 51 330445.0 52 322097.5 53 315295.5 54 308791.0 55 293306.5 56 269969.5 57 248055.0 58 228082.0 59 201047.0 60 166021.5 61 134698.0 62 110482.5 63 86882.0 64 64801.0 65 48367.0 66 36086.0 67 28123.5 68 21914.0 69 16371.5 70 12737.0 71 9846.0 72 7731.5 73 6114.5 74 4710.5 75 3530.5 76 2442.0 77 1524.0 78 929.5 79 587.0 80 394.0 81 260.0 82 165.0 83 115.5 84 83.5 85 63.5 86 46.5 87 30.5 88 27.5 89 20.5 90 10.5 91 5.5 92 3.0 93 2.5 94 1.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04308913894445836 2 0.010144095345455639 3 0.0 4 2.1272021694271323E-4 5 5.05210515238944E-4 6 0.0 7 0.0 8 3.9885040676758736E-5 9 0.0 10-11 6.6475067794597886E-6 12-13 3.057853118551503E-4 14-15 0.012803098057239552 16-17 0.0015821066135114298 18-19 5.318005423567831E-5 20-21 9.4394596268329E-4 22-23 7.046357186227376E-4 24-25 0.004433887021899679 26-27 0.016372809197809462 28-29 0.013474496241964992 30-31 0.009864900060718327 32-33 0.007152717294698734 34-35 0.008854479030240439 36-37 0.004846032442226186 38-39 0.004021741601573172 40-41 0.0054509555591570275 42-43 0.006820341955725744 44-45 0.006986529625212239 46-47 0.01584765616223214 48-49 0.018254053616396582 50-51 0.01766242551302466 52-53 0.011706259438628687 54-55 0.003975209054116954 56-57 0.003875496452425057 58-59 0.010130800331896719 60-61 0.002492815042297421 62-63 1.4624514914811536E-4 64-65 2.7919528473731113E-4 66-67 0.0010170685372573476 68-69 5.318005423567831E-5 70-71 1.26302628809736E-4 72-73 0.0031043856660077215 74-75 0.005590553201525683 76-77 0.005477545586274866 78-79 0.0035431211134520674 80-81 3.9885040676758736E-5 82-83 9.306509491243704E-4 84-85 2.991378050756905E-4 86-87 3.45670352531909E-4 88-89 1.5954016270703495E-4 90-91 4.2544043388542647E-4 92-93 4.5867796778272543E-4 94-95 1.9942520338379368E-5 96-97 0.0018347118711309017 98-99 4.5867796778272543E-4 100 1.0636010847135662E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7521617.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.96701972574809 #Duplication Level Percentage of deduplicated Percentage of total 1 72.03553200131695 38.15507444468512 2 16.142840415134334 17.10076293396047 3 5.353254429810617 8.50637798942182 4 2.307037287949374 4.887875581554034 5 1.1794126663188875 3.1234986980854833 6 0.6755081475059384 2.146779202431035 7 0.44520336378765885 1.6506766746197232 8 0.3094635196925239 1.3113088281562677 9 0.23678869890145976 1.1287792516993058 >10 1.1880389352383631 11.241380902875537 >50 0.07945995254983074 2.8978440442794224 >100 0.04325959196689194 4.198580354153397 >500 0.0028006668751058613 0.9876371465823425 >1k 0.0012295518602651748 1.262624462875228 >5k 6.830843667528448E-5 0.26573093680468346 >10k+ 1.0246265501292672E-4 1.1350685478162612 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25913 0.344513686352283 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22511 0.2992840502248386 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14320 0.19038459416372835 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9408 0.12507948756231538 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.3295013558919579E-5 2 0.0 0.0 0.0 0.0 1.3295013558919579E-5 3 0.0 0.0 0.0 0.0 1.3295013558919579E-5 4 0.0 0.0 0.0 2.6590027117839158E-5 1.3295013558919579E-5 5 0.0 0.0 0.0 2.6590027117839158E-5 1.3295013558919579E-5 6 0.0 0.0 0.0 2.6590027117839158E-5 2.6590027117839158E-5 7 0.0 0.0 0.0 2.6590027117839158E-5 2.6590027117839158E-5 8 0.0 0.0 0.0 3.9885040676758736E-5 6.647506779459789E-5 9 0.0 0.0 0.0 3.9885040676758736E-5 1.196551220302762E-4 10-11 0.0 0.0 0.0 3.9885040676758736E-5 1.196551220302762E-4 12-13 0.0 0.0 0.0 3.9885040676758736E-5 1.3295013558919577E-4 14-15 0.0 0.0 0.0 3.9885040676758736E-5 2.393102440605524E-4 16-17 0.0 0.0 0.0 4.653254745621852E-5 2.7919528473731113E-4 18-19 0.0 0.0 0.0 6.647506779459789E-5 3.390228457524492E-4 20-21 0.0 0.0 0.0 1.661876694864947E-4 3.589653660908286E-4 22-23 0.0 0.0 0.0 5.517430626951625E-4 4.254404338854265E-4 24-25 0.0 0.0 0.0 0.0014956890253784525 4.653254745621852E-4 26-27 0.0 0.0 0.0 0.0026789452321222948 4.786204881211048E-4 28-29 0.0 0.0 0.0 0.006713981847254387 5.052105152389439E-4 30-31 0.0 0.0 0.0 0.019457252343478802 5.318005423567831E-4 32-33 0.0 0.0 0.0 0.04936438534426839 5.98275610151381E-4 34-35 0.0 0.0 0.0 0.09448101385646199 6.248656372692202E-4 36-37 0.0 0.0 0.0 0.16538996867295955 6.248656372692202E-4 38-39 0.0 6.647506779459789E-6 0.0 0.2737509766849336 6.381606508281398E-4 40-41 0.0 1.3295013558919579E-5 0.0 0.4052320132758687 6.514556643870594E-4 42-43 0.0 1.3295013558919579E-5 0.0 0.526436004385759 6.514556643870594E-4 44-45 0.0 1.3295013558919579E-5 0.0 0.6691779174610991 6.713981847254386E-4 46-47 0.0 1.3295013558919579E-5 0.0 0.836163287761129 7.578157728584159E-4 48-49 0.0 1.3295013558919579E-5 0.0 1.014801737445552 7.977008135351747E-4 50-51 0.0 1.3295013558919579E-5 0.0 1.1984989397891437 7.977008135351747E-4 52-53 0.0 1.3295013558919579E-5 0.0 1.385699378205511 9.040609220065313E-4 54-55 0.0 1.3295013558919579E-5 0.0 1.6040965659378827 0.001003773523698428 56-57 0.0 1.3295013558919579E-5 0.0 1.845081981706859 0.0010237160440368074 58-59 0.0 1.3295013558919579E-5 0.0 2.1203884749781863 0.0010436585643751868 60-61 0.0 1.3295013558919579E-5 0.0 2.40188379706119 0.0010569535779341064 62-63 0.0 1.3295013558919579E-5 0.0 2.686250842072921 0.001103486125390325 64-65 0.0 1.3295013558919579E-5 0.0 2.976760183348873 0.0011167811389492445 66-67 0.0 1.3295013558919579E-5 0.0 3.3012981118288796 0.001136723659287624 68-69 0.0 1.3295013558919579E-5 0.0 3.6824993880970007 0.0011433711660670837 70-71 0.0 1.3295013558919579E-5 0.0 4.098726377586097 0.0011766086999643827 72-73 0.0 1.3295013558919579E-5 0.0 4.468806375012182 0.001415918944024935 74-75 0.0 1.3295013558919579E-5 0.0 4.837171315689167 0.001449156477922234 76-77 0.0 1.3295013558919579E-5 0.0 5.247734629402162 0.0014757465050400731 78-79 0.0 1.3295013558919579E-5 0.0 5.717640236135395 0.001482394011819533 80-81 0.0 1.3295013558919579E-5 0.0 6.187080517394065 0.00157545910673197 82-83 0.0 1.3295013558919579E-5 0.0 6.671996194435319 0.001728351762659545 84-85 0.0 1.3295013558919579E-5 0.0 7.165905948149181 0.001728351762659545 86-87 0.0 1.3295013558919579E-5 0.0 7.681114313584432 0.001728351762659545 88 0.0 1.3295013558919579E-5 0.0 8.094974790660041 0.001728351762659545 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 15435 0.0 61.16915 2 GTACCTG 17700 0.0 53.89087 1 ACCTGGG 17540 0.0 53.234806 3 CCTGGGG 14945 0.0 43.800854 4 GGTATCA 18470 0.0 35.914227 1 TATAACG 925 0.0 35.564396 2 CTGGGGG 9075 0.0 34.176273 5 GTATCAA 28590 0.0 32.492207 1 TAACGCA 1055 0.0 31.625202 4 ATAACGC 1120 0.0 30.628958 3 GTACCCG 1635 0.0 30.191034 1 TGGGGGG 13795 0.0 28.614422 6 TCAACGC 34795 0.0 26.308706 4 CAACGCA 35400 0.0 25.898907 5 TATCAAC 35575 0.0 25.773388 2 ATCAACG 35530 0.0 25.764463 3 GTACATG 32375 0.0 25.615051 1 AACGCAG 36785 0.0 25.102629 6 CATGGGG 21465 0.0 24.82616 4 TACATGG 33220 0.0 24.629654 2 >>END_MODULE