FastQCFastQC Report
Mon 6 Jun 2016
ERR523205_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523205_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13973680
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470870.33696921641256994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT382790.2739364290580577No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210080.15033978164663855No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT159530.11416462950346652No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG203850.049.8754272
ACCTGGG229950.042.8848153
GTACCTG273150.037.566021
TATAACG14050.032.8013272
CCTGGGG204950.030.9758224
TATCACG12500.028.5920452
TAACGCA16450.028.5871664
ATAACGC16000.028.5095443
GGTATCA447300.028.0812641
GTATCAA631950.027.5408941
GTACATG496650.026.1904741
TACATGG508150.025.1535422
ACATGGG510200.024.0291883
CATGGGG338800.023.9432284
TCAACGC718800.023.9251084
CAACGCA727700.023.6186395
ATGGGGG230400.023.4916065
ATCAACG731700.023.4841043
TATCAAC742350.023.2107092
AACGCAG750850.023.0257386
Produced by FastQC (version 0.11.5)