FastQCFastQC Report
Mon 6 Jun 2016
ERR523205_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523205_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13973680
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT436050.3120509414842762No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT376500.26943510943430793No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204240.14616049601822856No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT162470.11626858493968661No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG200650.050.9726262
ACCTGGG224000.044.3566473
GTACCTG271150.038.215891
TATAACG14750.035.0524142
ATAACGC16300.032.583113
CCTGGGG197800.031.602914
TAACGCA17000.029.8590894
GGTATCA466200.026.3737831
GTACATG489950.026.1993711
TACATGG501250.025.1771852
GTATCAA637350.025.1144141
CTGGGGG129950.024.233225
ACATGGG499350.024.1143653
CATGGGG324150.024.0693194
TGGGGGG221900.023.7642066
TATCACG11300.023.2930762
ATGGGGG215450.023.0372185
TCAACGC716200.022.0564654
CAACGCA722850.021.8606035
ATCAACG729000.021.6820873
Produced by FastQC (version 0.11.5)