FastQCFastQC Report
Mon 6 Jun 2016
ERR523204_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523204_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9707130
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT408430.42075258083491207No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327260.33713363270091157No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213400.21983840743865593No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186470.19209591300415263No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA126270.1300796424895927No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT98710.1016881405729603No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG201250.059.577632
ACCTGGG229050.051.7521253
GTACCTG235750.051.4705961
CCTGGGG203400.042.370714
TATAACG9550.034.9569782
CTGGGGG125250.032.4697655
TGGGGGG217700.028.55976
ATGGGGG196250.026.4005345
TATCACG9150.026.2076442
CATGGGG299550.026.1963794
ATAACGC13650.026.1798153
TAACGCA14200.026.1573034
GTACGTT8750.024.7252661
GTACATG530800.024.4373741
GTACCCG21950.024.4265161
TACATGG548800.023.4155542
TACGTTG9650.022.9007362
ACATGGG550900.022.6523783
GTATCAA564450.022.522251
GGTATCA434850.021.2527071
Produced by FastQC (version 0.11.5)