FastQCFastQC Report
Mon 6 Jun 2016
ERR523204_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523204_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9707130
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT359740.37059357400179044No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT318810.328428691075529No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205780.21198850741671327No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169840.1749641758171571No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA122090.12577352935419636No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT106890.11011493613457325No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG212100.058.6320882
ACCTGGG236650.051.629673
GTACCTG246950.050.746361
CCTGGGG208550.042.294764
CTGGGGG121950.034.8352665
TATAACG9250.032.5180472
TGGGGGG191100.030.04996
GTACCCG19650.026.8023071
TATCACG9450.025.8617422
TACCGTA9500.025.7225767
TAACGCA12750.025.0628974
ATGGGGG182700.024.7953935
CATGGGG292450.024.344094
GTACACG20650.023.2271981
GTACATG517850.022.9829031
ATAACGC13450.022.7103373
TACATGG534550.022.0244752
GTTAAGA75650.021.4310114
ACATGGG534150.021.3344293
GTGGTTA61600.021.2217311
Produced by FastQC (version 0.11.5)