FastQCFastQC Report
Mon 6 Jun 2016
ERR523203_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523203_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15756027
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT452350.2870964869506761No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT374110.23743929862521815No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215460.1367476712244781No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG181850.038.452
GTACCTG220300.032.473021
ACCTGGG227450.030.2452413
GTACATG754050.025.4613111
GTATCAA682400.024.778091
CATGGGG457000.024.7434484
TACATGG777400.024.3879152
GGTATCA500650.024.1814441
ACATGGG788750.023.4348583
ATGGGGG264400.022.9021155
TCAACGC771350.021.5232354
CAACGCA774400.021.4364855
ATCAACG786200.021.0826263
AACGCAG792150.021.0531236
TATCAAC797950.020.8723582
CCTGGGG231550.020.833584
GAGTACT394800.020.06693812-13
ACGCAGA882350.018.8424037
TGGGGGG248100.018.7889676
TATAACG12350.018.2756562
Produced by FastQC (version 0.11.5)