FastQCFastQC Report
Mon 6 Jun 2016
ERR523203_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523203_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15756027
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT402880.2556989779212742No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT359000.22784931759764057No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213060.13522444458872784No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG184750.040.2954442
GTACCTG224250.033.42171
ACCTGGG226800.032.1370283
GTATCAA693550.024.1416451
GGTATCA515000.023.5916291
GTACATG751000.023.434331
CATGGGG444750.023.2985864
TACATGG776000.022.4091422
ACATGGG772350.021.952773
ATGGGGG255500.021.7769855
CCTGGGG233850.021.4620324
CAACGCA789650.020.7636325
TCAACGC789150.020.758924
TGGGGGG228350.020.3737566
ATCAACG805250.020.3555453
TATCAAC814250.020.2482262
AACGCAG813850.020.1981876
GAGTACT404000.019.80221212-13
GTACTTT443400.018.03556414-15
ACGCAGA912550.018.0084327
Produced by FastQC (version 0.11.5)