FastQCFastQC Report
Mon 6 Jun 2016
ERR523202_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523202_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5040786
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT353080.7004463192843339No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181010.3590908243277933No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141670.28104743982386876No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT98280.1949695940275981No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81260.16120501842371407No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC81050.16078841672707392No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC71330.14150570962544334No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA58660.11637074059481993No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG56150.11139135841116841No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG176500.027.0144461
TACATGG180300.026.2620092
ACATGGG183500.024.8059063
TACCTGG38450.024.7029722
CATGGGG123300.024.2899884
ATGGGGG75350.021.5280195
GAGTACT156550.020.91598312-13
GTACCTG48050.020.3550781
AGAGTAC237300.019.79706410-11
AGTACTT166050.018.88473712-13
GTACTTT172650.018.84344914-15
ACCTGGG49550.017.8414443
TGGGGGG73900.016.4028456
GAGTACA137800.016.0039221
ACTTTTT210050.015.70016916-17
TACTTTT202500.015.45099314-15
GTATAAT23650.015.3095491
GTATTAG15200.015.1584681
CATGGGA62850.015.1111334
AGTACAT135450.014.9620962
Produced by FastQC (version 0.11.5)