FastQCFastQC Report
Mon 6 Jun 2016
ERR523202_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523202_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5040786
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT351030.6963794931980846No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159180.3157840860532465No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133420.2646809445987193No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT98510.19542587207629922No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC80880.1604511677345557No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76210.1511867395283196No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC71810.14245794207490656No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG55010.10912980634369322No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA52530.10420993868813315No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG180600.026.669741
TACATGG185500.025.6674772
TACCTGG37900.024.8032422
ACATGGG187100.024.6904183
CATGGGG126050.023.040634
ATGGGGG75200.021.7473245
TATAACG4050.020.8898412
GAGTACT150400.020.67650812-13
GTACCTG47850.020.4464551
TAACGCA5500.019.652324
GTACTTT167450.018.45633514-15
AGAGTAC236050.018.33212710-11
ACCTGGG50100.018.2913443
TATCACG4700.018.000822
TGGGGGG70650.017.9595746
AGTACTT159200.017.21554412-13
CATGGGA62950.016.7224924
ATAACGC5400.016.5351663
GAGTACA141450.015.7287711
CTATGCG3957.508788E-915.471219
Produced by FastQC (version 0.11.5)