FastQCFastQC Report
Mon 6 Jun 2016
ERR523201_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523201_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12482177
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261950.2098592256783412No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200770.16084533971918522No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA195350.038.0108761
GTATCAA321150.036.1326181
TCAACGC371600.030.5289754
ATCAACG379200.029.9317843
CAACGCA380600.029.8672395
AACGCAG396800.028.807536
TATCAAC398250.028.6218242
GTACATG383500.027.4114551
TACATGG397450.025.6400072
ACGCAGA452450.025.2226527
TACCTGG105150.024.2734722
CGCAGAG472100.024.072058
ACATGGG407900.024.0234953
CATGGGG280950.021.3772814
GCAGAGT528850.021.061849
GTACCTG132300.020.1312561
AGAGTAC432850.019.36684810-11
GAGTACT268200.019.05056412-13
ACCTGGG135200.018.0763383
CAGAGTA513500.017.58756410-11
Produced by FastQC (version 0.11.5)