FastQCFastQC Report
Mon 6 Jun 2016
ERR523201_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523201_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12482177
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241130.19317944297697429No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197750.1584258899709562No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA198500.038.1794621
GTATCAA319650.034.8793531
TCAACGC374550.029.1086544
CAACGCA383450.028.4207785
ATCAACG383150.028.3700083
AACGCAG397250.027.527456
TATCAAC402000.027.2583332
GTACATG376150.025.2755151
TACCTGG104150.024.2936292
TACATGG386450.024.1323052
ACGCAGA460350.023.6621177
ACATGGG397400.022.717063
CGCAGAG481550.022.5961068
CATGGGG270250.020.0322974
GCAGAGT545450.019.7249019
GTACCTG136200.019.2385751
AGAGTAC446600.018.86059810-11
GAGTACT272150.018.73099712-13
ACCTGGG130650.018.668553
CAGAGTA523500.017.13159810-11
Produced by FastQC (version 0.11.5)