FastQCFastQC Report
Mon 6 Jun 2016
ERR523200_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523200_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6240384
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223690.35845550530223785No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174330.27935780875023075No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115360.18486041884601973No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77970.12494423420097225No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT73990.11856642155354542No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG100150.050.9012262
ACCTGGG110050.045.6384773
GTACCTG117450.044.0439381
CCTGGGG99350.032.423934
TAACGCA7600.032.1760524
TATAACG7400.031.1396922
ATAACGC8200.029.8221823
GTATCAA270150.026.5815641
GGTATCA190750.025.9110261
GTACATG201050.025.682941
TACATGG202800.024.8354172
ACATGGG200800.024.1459853
CATGGGG113800.023.8438574
TCAACGC307550.022.9207524
TACCGGG8850.022.8494172
CAACGCA310950.022.6841645
TATCACG5600.022.6738722
ATCAACG311500.022.6002733
CTGGGGG64500.022.1633785
AACGCAG322250.022.0701056
Produced by FastQC (version 0.11.5)