FastQCFastQC Report
Mon 6 Jun 2016
ERR523200_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523200_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6240384
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217470.34848816995877174No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181190.29035072200685086No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117490.18827367033823558No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT79070.1267069462392058No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71140.11399939490903124No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG105550.053.262912
ACCTGGG113850.047.8919943
GTACCTG124600.045.376481
CCTGGGG99250.034.0988734
TAACGCA7800.030.733614
TATAACG7950.028.972162
ATAACGC8650.027.7135433
GTATCAA283700.025.052411
GGTATCA208050.024.6209351
GTACATG201900.022.69171
CTGGGGG64500.022.081115
TACATGG205000.021.8061282
TCAACGC323500.021.7367864
ACATGGG201400.021.5883883
ATCAACG328300.021.4046573
CAACGCA329200.021.3889755
TATCAAC335400.021.0363082
AACGCAG340600.020.8912076
TACCGGG8000.020.565062
CATGGGG119950.019.4757484
Produced by FastQC (version 0.11.5)