FastQCFastQC Report
Mon 6 Jun 2016
ERR523199_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523199_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7255343
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285820.39394415949735256No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237960.3279789804561962No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138070.1903011339367415No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT118620.16349330417597074No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81960.11296502453433284No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA75130.1035512724898051No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG103400.044.523672
GTACCTG118550.039.358191
ACCTGGG122250.036.542693
CCTGGGG115850.025.9759044
TATCACG7500.021.9452
GTACATG376500.021.8624111
CATGGGG195000.021.5329534
TACATGG385600.021.1466142
GAGTACT231550.020.4554812-13
GTATCAA485150.020.3886381
ACATGGG386900.020.3218563
TATAACG7050.020.010642
ATGGGGG111200.019.7878115
TAACGCA7750.018.8081784
AGTACTT245300.018.56180212-13
GGTATCA380750.018.4435631
TCAACGC539900.017.9930084
CAACGCA540700.017.9477525
AACGCAG550700.017.757776
GTACCCG13800.017.723491
Produced by FastQC (version 0.11.5)