FastQCFastQC Report
Mon 6 Jun 2016
ERR523199_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523199_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7255343
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251600.34677891865346683No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225170.31035059265978193No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133590.18412637417693417No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT122880.1693648391261447No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74300.10240728798073363No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA73770.101676791848435No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG104750.047.8730742
GTACCTG123550.041.027541
ACCTGGG124900.039.3928383
CCTGGGG115800.027.9198384
TATCACG6300.025.364092
TATAACG7450.023.972222
ATAACGC8000.021.7342553
CATGGGG195800.021.024444
TAACGCA8150.020.7576374
GTACATG366300.020.4878061
GAGTACT229300.019.99882312-13
TACATGG372150.019.865142
GTATCAA467350.019.861151
GTACACG16350.019.5564791
ATGGGGG114750.019.4524335
ACATGGG374050.019.0710723
TGGGGGG104050.018.9236536
GGTATCA369200.018.8749581
GTATAAC17850.017.9130761
AGTACTT241150.017.78798712-13
Produced by FastQC (version 0.11.5)