FastQCFastQC Report
Mon 6 Jun 2016
ERR523198_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523198_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11951343
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT325150.2720614745974574No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254270.21275433229554203No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138120.1155686017881003No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA178800.050.114891
GTATCAA329300.042.3597951
TCAACGC392750.034.7129864
ATCAACG399900.034.144073
CAACGCA400450.034.0214735
AACGCAG418450.032.6734626
TATCAAC419200.032.588482
ACGCAGA478900.028.480647
CGCAGAG493700.027.625358
GTACATG421700.024.9983771
GCAGAGT553100.024.3687119
TACATGG428600.024.0533892
ACATGGG439900.022.8868543
GAGTACT305200.020.93617812-13
CAGAGTA538800.020.48099510-11
AGAGTAC498600.020.31384510-11
GTACTTT322650.019.59952214-15
CATGGGG284550.019.554814
GTGGTAT106950.018.7894691
AGTACTT332550.017.97103912-13
Produced by FastQC (version 0.11.5)