FastQCFastQC Report
Mon 6 Jun 2016
ERR523198_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523198_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11951343
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288010.24098546916442778No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239820.20066364089793087No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132250.11065701988471086No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA182600.051.3982121
GTATCAA322450.040.4277951
TCAACGC391600.032.6404234
ATCAACG398850.032.0365373
CAACGCA399500.031.9596825
TATCAAC417250.030.9072672
AACGCAG417350.030.6600726
ACGCAGA481700.026.524977
CGCAGAG498900.025.5587948
GTACATG420950.024.3518871
TACATGG432800.023.2767162
GCAGAGT558150.022.685539
ACATGGG434500.022.5724453
GAGTACT305600.020.35542312-13
CATGGGG281500.019.9322134
AGAGTAC502150.019.87596910-11
CAGAGTA541650.019.84960710-11
GTACTTT321550.019.1100514-15
AGTACTT330600.017.4299912-13
GAGTACA341500.016.9579451
Produced by FastQC (version 0.11.5)