FastQCFastQC Report
Mon 6 Jun 2016
ERR523197_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523197_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6481562
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263430.4064298081234122No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214120.33035246750706077No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129290.1994735219689328No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108140.16684249876804386No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT77510.11958537155087No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG110850.053.531762
GTACCTG124650.048.221881
ACCTGGG125750.046.366933
CCTGGGG110200.035.4117474
TATAACG7350.035.185462
ATAACGC8800.030.991313
TAACGCA8700.030.8039574
GTACCCG11450.025.8785921
CTGGGGG72400.024.6103575
GTATCAA372550.023.1915721
CATGGGG159150.022.599864
GTACATG291350.022.003231
GGTATCA279750.021.8732241
TACCCGG13600.021.4358462
TACATGG299050.021.0849632
TACCGGG8450.020.5888882
ACATGGG297200.020.5362193
GAGTACT222000.020.50094812-13
TCAACGC415300.020.4798854
ATGGGGG96350.020.4446435
Produced by FastQC (version 0.11.5)