FastQCFastQC Report
Mon 6 Jun 2016
ERR523197_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523197_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6481562
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238070.36730343704187357No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212010.3270970793768539No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124990.1928393186704069No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95420.1472175997082185No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT80500.12419845710031008No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG113250.051.7081452
GTACCTG129700.045.390471
ACCTGGG126850.045.0846753
CCTGGGG113350.033.6223564
TATAACG6350.030.3451462
ATAACGC7350.026.2135223
GTACCCG11300.023.7190191
GGTATCA287150.022.991041
CTGGGGG71950.022.9900915
GTATCAA374650.022.0644681
TGGGGGG95900.021.266866
GTACATG281300.021.1456531
TATCACG6450.021.130872
TAACGCA9000.020.8855694
CATGGGG156950.020.7791124
ATGGGGG94050.020.2859865
GAGTACT231700.020.17459312-13
TACATGG290050.020.1732392
ACATGGG290050.019.506643
TCAACGC422700.019.2995114
Produced by FastQC (version 0.11.5)