FastQCFastQC Report
Mon 6 Jun 2016
ERR523196_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523196_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4663575
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165350.3545563221348429No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133540.2863468476437068No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74760.16030620285939434No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT55200.11836413052218524No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG61250.043.533872
ACCTGGG68300.038.007453
GTACCTG73500.037.2375641
GGTATCA156850.027.8832971
GTACATG180350.026.5446681
GTATCAA215100.025.9948391
CCTGGGG68900.025.800194
TACATGG183550.025.1598152
CATGGGG119450.024.8423984
ACATGGG180900.024.462533
TATAACG4500.024.036192
ATGGGGG73850.022.2234385
TCAACGC247450.022.2165874
CAACGCA250450.021.9122095
ATCAACG253100.021.7763843
TATCAAC254950.021.7290422
TATCACG4150.021.5305842
AACGCAG258350.021.4338726
GAGTACT143700.020.4267812-13
TAACGCA5550.019.4888044
Produced by FastQC (version 0.11.5)