FastQCFastQC Report
Mon 6 Jun 2016
ERR523196_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523196_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4663575
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153840.3298756854987858No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135120.2897348064521317No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75550.16200018226360677No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT58620.12569756034801627No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG60850.041.403612
ACCTGGG66900.036.88453
GTACCTG72500.035.3536871
TATAACG4350.025.933192
CCTGGGG69150.025.1489724
GTACATG175900.024.9454881
GGTATCA168150.024.8925481
TACATGG177900.024.281392
GTATCAA228400.024.1328011
TAACGCA5950.023.698184
ACATGGG177500.023.5933723
CATGGGG115000.022.5606654
TCAACGC257400.021.3094884
CAACGCA259500.021.173725
ATGGGGG68850.021.1630255
ATAACGC6250.021.0566233
ATCAACG263450.020.7487623
AACGCAG267650.020.6679136
GAGTACT141850.020.6240612-13
TATCAAC268750.020.3581582
Produced by FastQC (version 0.11.5)