FastQCFastQC Report
Mon 6 Jun 2016
ERR523195_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523195_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5020981
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205340.40896390565907337No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167640.3338789770365592No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97880.19494198444487243No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82530.16437026947522806No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG78200.050.7548332
GTACCTG91350.043.872261
ACCTGGG91050.042.7141383
TATAACG5100.035.039282
GGTATCA153650.032.84931
CCTGGGG81700.032.1733864
GTATCAA225550.030.6364151
ATAACGC6100.030.066363
TAACGCA6800.026.9688664
TCAACGC261050.026.1727284
ATCAACG263850.025.8081913
CAACGCA264250.025.7466965
TATCAAC266850.025.623532
AACGCAG272350.025.1562826
CTGGGGG56250.023.4047225
GTACATG213650.023.3599261
ACGCAGA300350.022.7324167
CATGGGG133150.022.6019464
TACATGG216950.022.391462
CGCAGAG309500.022.0900658
Produced by FastQC (version 0.11.5)