FastQCFastQC Report
Mon 6 Jun 2016
ERR523195_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523195_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5020981
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186010.3704654528666808No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168100.334795132664314No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95940.19107819766694994No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71560.14252194939594473No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG81200.053.136922
GTACCTG92350.047.2561951
ACCTGGG94100.045.195493
CCTGGGG83550.032.5100484
GGTATCA157450.030.5847551
GTATCAA223150.027.6493321
TATAACG4700.025.0006832
GTACATG218900.023.5027831
CATGGGG129300.023.3694574
TCAACGC261700.023.2003734
CAACGCA266100.022.8520725
ATCAACG265850.022.8205343
TATCAAC267750.022.8204172
CTGGGGG55250.022.70995
TACATGG224450.022.5321372
AACGCAG273050.022.4250856
ACATGGG225000.021.8257883
ATAACGC5650.021.6252483
TAACGCA5650.020.7935074
ATGGGGG81750.020.579345
Produced by FastQC (version 0.11.5)