FastQCFastQC Report
Mon 6 Jun 2016
ERR523194_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523194_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11978810
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT554000.46248333515599627No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT399300.33333862044727314No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT341610.2851785778387002No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187770.15675179754917223No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA175190.14624991964978157No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT134970.11267396344044191No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG121350.033.2479062
GTACCTG151000.027.6923561
ACCTGGG150250.026.0709253
CATGGGG315850.021.9422234
GAGTACT338400.020.64154412-13
GTACATG672750.020.303831
ATGGGGG182350.019.774625
TACATGG687300.019.5401842
ACATGGG680050.019.1404063
GTACTTT380850.018.29218914-15
AGTACTT368400.017.3534812-13
CCTGGGG150550.016.3649444
ACTTTTT460550.015.47851116-17
TGGGGGG176250.015.4370516
AGAGTAC770850.014.15003110-11
GTACCGT13650.014.1145216
TATAACG9350.014.0819642
TACTTTT443900.014.07434214-15
GTATAGG36950.014.0027491
ACTATAC37000.013.7261253
Produced by FastQC (version 0.11.5)