FastQCFastQC Report
Mon 6 Jun 2016
ERR523194_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523194_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11978810
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT556470.46454530959252216No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT349440.2917151202832335No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT332410.27749834916823957No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184580.15408876173843644No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA169830.1417753516417741No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT127330.1062960344141029No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG123900.034.2531742
GTACCTG149900.028.9224381
ACCTGGG156050.026.3500543
GAGTACT355750.020.560712-13
CATGGGG313050.019.695064
GTATAGG33250.018.9504171
GTACATG677000.018.6978651
GTACTTT390850.018.54949214-15
TACATGG694450.018.0157132
AGTACTT381250.017.74904612-13
ACATGGG683250.017.717543
ATGGGGG179300.017.6126375
CCTGGGG143000.017.154254
TATCACG9050.017.1375892
GTACACG26750.017.0511461
ACTTTTT473450.015.61913816-17
GTATAGA38300.015.1012151
TGGGGGG157850.014.5876426
TACTTTT457750.014.54984914-15
AGAGTAC802000.014.02021210-11
Produced by FastQC (version 0.11.5)