FastQCFastQC Report
Mon 6 Jun 2016
ERR523193_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523193_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7314992
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188960.2583188060902869No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145020.198250387696938No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82940.11338358264780057No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98600.050.531411
GTATCAA180500.041.157341
TCAACGC213450.034.0146144
CAACGCA217600.033.3663565
ATCAACG217800.033.3364033
TATCAAC225650.032.3054052
AACGCAG226050.032.133056
ACGCAGA263850.027.4761437
CGCAGAG272150.026.619828
GTACATG217350.025.8023361
TACATGG220200.024.5032272
ACATGGG223700.023.5078123
GCAGAGT307450.023.2110359
TACCTGG64850.022.7571752
GTGGTAT54350.021.208451
CATGGGG156150.021.0964454
GAGTACT186400.020.7320112-13
GTACCTG75400.020.5289611
CAGAGTA298350.019.92670210-11
AGAGTAC280700.019.4307310-11
Produced by FastQC (version 0.11.5)