FastQCFastQC Report
Mon 6 Jun 2016
ERR523193_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523193_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7314992
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169590.23183894117724257No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143450.1961041105718229No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78640.10750524402487385No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96950.050.6622121
GTATCAA174350.040.449311
TCAACGC207950.033.332554
ATCAACG211850.032.720263
CAACGCA213950.032.353845
TATCAAC220250.031.7520682
AACGCAG223450.031.0620166
ACGCAGA258250.026.8943217
CGCAGAG267000.025.9480538
GTACATG220650.025.0533241
TACATGG225300.023.692542
TACCTGG61550.023.5342062
ACATGGG225300.023.0491183
GCAGAGT304650.022.4944259
CATGGGG155900.021.6428154
GTACCTG74150.020.303471
GAGTACT179950.020.08625612-13
CAGAGTA293750.019.54519810-11
AGAGTAC275550.019.41182310-11
GTACTTT196900.018.3074614-15
Produced by FastQC (version 0.11.5)