FastQCFastQC Report
Mon 6 Jun 2016
ERR523192_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523192_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6112536
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180700.29562198079487795No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT179830.29419867629409463No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135730.22205186194404417No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76460.12508719785045028No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG73600.035.7180562
GTACCTG86500.030.9352441
ACCTGGG87650.028.9197273
GTACATG224600.025.0425621
TACATGG227800.024.504782
ACATGGG231150.023.1936073
CATGGGG149750.021.4802594
GAGTACT159650.020.48914712-13
TATAACG5700.019.8011382
ATGGGGG84450.019.3775045
CCTGGGG86950.019.2544294
GTATAGG17750.019.0761831
GTATCAA402600.018.9233761
GTACTTT176050.018.43447314-15
AGAGTAC278100.018.15753710-11
AGTACTT169650.018.00706912-13
TCAACGC430950.017.4272274
CAACGCA432300.017.349775
ATCAACG438900.017.1011263
AACGCAG442800.017.0057326
Produced by FastQC (version 0.11.5)