FastQCFastQC Report
Mon 6 Jun 2016
ERR523192_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523192_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6112536
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT184430.30172419434421327No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171700.28089814113160233No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138940.22730336475727914No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78150.1278520077427765No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG77550.037.2766652
GTACCTG88550.033.4647181
ACCTGGG88000.031.9407023
GTACATG218400.023.453651
TACATGG224150.022.4592082
ACATGGG228600.021.383713
CATGGGG145050.020.8362314
CCTGGGG90250.020.676134
GAGTACT168200.020.08979612-13
TATAACG4700.019.0020072
AGTACTT173100.018.8966512-13
GTACTTT178150.018.8752614-15
AGAGTAC283300.018.19023310-11
GTATCAA401600.017.826091
ATGGGGG82600.017.4699385
ATAACGC6400.016.8917163
GTATAGG16150.016.3098751
TACTTTT201550.016.21748214-15
TCAACGC435900.016.0989994
CAACGCA437450.016.0637075
Produced by FastQC (version 0.11.5)