FastQCFastQC Report
Mon 6 Jun 2016
ERR523191_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523191_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7176900
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT868321.2098817037996907No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT253650.3534255737156711No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206530.2877704858643704No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC192500.26822165558946065No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC177990.24800401287463947No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163020.22714542490490322No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG140850.19625465033649625No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA140180.19532109963911995No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA123200.1716618595772548No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC109930.15317198233220472No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93530.13032089063523247No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG78690.10964343936797223No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG278050.027.5964971
TACATGG284150.026.6372452
ACATGGG290500.025.4257973
CATGGGG205050.024.371294
TACCTGG47600.022.9135382
ATGGGGG125150.022.4643365
GAGTACT184100.020.1094212-13
AGAGTAC318100.018.3578110-11
GTACTTT199300.018.35185214-15
AGTACTT192150.018.22780212-13
GTACCTG65850.017.3503071
TGGGGGG119900.017.2030056
GAGTACA216050.016.9745031
AGTACAT210450.016.2403962
GTATAGG18450.016.0546511
CATGGGA93450.015.8969124
GTGTAGC33950.015.5108261
GTATAAT29350.015.3787071
ACTTTTT250400.014.96290316-17
TACTTTT230950.014.84007114-15
Produced by FastQC (version 0.11.5)