##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR523191_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7176900
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.906385765441904	34.0	31.0	34.0	30.0	34.0
2	32.209118700274495	34.0	31.0	34.0	30.0	35.0
3	32.05865568699578	34.0	31.0	34.0	28.0	35.0
4	34.66679722442837	35.0	35.0	37.0	32.0	37.0
5	34.67602000863883	37.0	34.0	37.0	32.0	37.0
6	34.85265532472237	37.0	35.0	37.0	33.0	37.0
7	34.70274756510471	37.0	35.0	37.0	32.0	37.0
8	34.84374353829648	37.0	35.0	37.0	32.0	37.0
9	36.08958254956875	39.0	35.0	39.0	32.0	39.0
10-11	36.188635204614805	38.0	35.5	39.0	32.5	39.0
12-13	36.23783374437431	38.0	35.5	39.0	32.5	39.0
14-15	37.31634577603144	39.0	36.5	41.0	32.0	41.0
16-17	37.21562324959244	39.0	36.5	41.0	32.0	41.0
18-19	37.23926089258593	39.0	36.5	41.0	32.5	41.0
20-21	37.252759408658335	39.0	36.0	41.0	32.0	41.0
22-23	37.15964385737575	39.0	36.0	41.0	32.0	41.0
24-25	37.150524042413856	39.0	36.5	41.0	32.0	41.0
26-27	36.9709953461801	39.0	36.5	41.0	32.0	41.0
28-29	36.78155025150134	39.0	35.5	41.0	31.0	41.0
30-31	36.70460707269155	38.0	36.0	41.0	30.5	41.0
32-33	36.52711100893143	38.0	35.0	41.0	30.5	41.0
34-35	36.41355863952403	38.5	35.0	41.0	30.0	41.0
36-37	36.2949632153158	38.0	35.5	40.5	30.0	41.0
38-39	36.051246847524695	38.0	35.5	40.0	30.0	41.0
40-41	36.01699849517201	38.0	35.0	40.0	30.0	41.0
42-43	35.970301662277585	38.0	35.0	40.0	29.0	41.0
44-45	35.864732126684224	38.0	35.0	40.0	29.0	41.0
46-47	35.57700413827697	38.0	35.0	40.0	28.0	41.0
48-49	35.68760913486324	37.5	35.0	40.0	28.0	41.0
50-51	34.654259220554835	37.5	34.0	39.0	26.0	40.0
52-53	34.82890739734426	37.0	34.5	39.5	26.5	40.0
54-55	35.41256949379259	37.0	34.5	40.0	28.0	41.0
56-57	35.539844570775685	37.0	35.0	40.0	28.0	41.0
58-59	35.51970600119829	38.0	35.0	40.0	27.5	41.0
60-61	35.224600663239	37.0	35.0	40.0	27.0	41.0
62-63	35.02281138095836	37.0	34.0	40.0	26.5	41.0
64-65	34.78233958951637	36.5	34.0	39.5	26.5	41.0
66-67	34.517459348743884	37.0	34.0	39.0	26.0	41.0
68-69	34.160434240410204	36.0	34.0	38.5	26.0	41.0
70-71	33.73219816355251	36.0	33.5	37.5	25.0	40.0
72-73	33.470471373434215	35.5	33.5	37.0	25.0	39.5
74-75	33.22285276372807	35.0	33.0	37.0	24.0	39.0
76-77	32.88966099569452	35.0	33.0	36.5	24.5	39.0
78-79	32.58294772116095	35.0	33.0	37.0	23.5	37.5
80-81	32.35153499421756	35.0	32.0	36.0	22.5	37.5
82-83	32.13131226574147	35.0	32.0	36.0	21.5	37.0
84-85	31.844310147835415	35.0	32.0	35.5	20.5	37.0
86-87	31.780895233318006	35.0	32.0	36.0	20.0	37.0
88-89	31.471231311569063	35.0	31.5	35.0	19.5	37.0
90-91	31.36596079087071	35.0	31.0	35.0	19.0	36.5
92-93	31.126460101157882	35.0	31.0	35.0	18.5	36.0
94-95	31.024114868536554	34.5	31.0	35.0	18.5	36.0
96-97	30.760180997366554	34.0	30.5	35.0	19.0	36.0
98-99	30.71818835430339	34.0	30.0	35.0	18.5	36.5
100	29.459784168652202	34.0	27.0	35.0	17.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.005755608071172702
1101	2	-0.053512377728090144
1101	3	-0.017658705629365556
1101	4	-0.07203635288630039
1101	5	-0.03792700511600344
1101	6	-0.08333531096962332
1101	7	0.11562673744720797
1101	8	-0.025124194357275087
1101	9	-0.17242722839115032
1101	10-11	-0.10942506497468685
1101	12-13	-0.13435043224611576
1101	14-15	-0.03418074097575641
1101	16-17	-0.1993089657588527
1101	18-19	-0.1605132376444729
1101	20-21	-0.13079359379886313
1101	22-23	-0.2006186105153347
1101	24-25	-0.28878216737722084
1101	26-27	-0.34421724792971986
1101	28-29	-0.29095399593254
1101	30-31	-0.28846748495831065
1101	32-33	-0.27542842562334613
1101	34-35	-0.4030057636794737
1101	36-37	-0.28859560985062416
1101	38-39	-0.38934867042939914
1101	40-41	-0.3266284111560154
1101	42-43	-0.24908467232251752
1101	44-45	-0.4064845572705664
1101	46-47	-0.3798099732500688
1101	48-49	-0.41477180558291593
1101	50-51	-0.578391193739769
1101	52-53	-0.45022465448981563
1101	54-55	-0.3451006855827927
1101	56-57	-0.2151113188708038
1101	58-59	-0.19028352482987998
1101	60-61	-0.16674536554556596
1101	62-63	-0.1926078877057975
1101	64-65	-0.1643023932204244
1101	66-67	-0.4937319522500232
1101	68-69	-0.31720633676519583
1101	70-71	-0.21677957066300024
1101	72-73	-0.2938731097597014
1101	74-75	-0.388277558264555
1101	76-77	-0.3647876846265987
1101	78-79	-0.5691954189414332
1101	80-81	-0.3310198780739455
1101	82-83	-0.396594948580983
1101	84-85	-0.7380132983288341
1101	86-87	-0.9642972256625271
1101	88-89	-0.4541699604528837
1101	90-91	-0.6489938558682091
1101	92-93	-0.4771871817949389
1101	94-95	-0.6715867134820712
1101	96-97	-0.6570960739503491
1101	98-99	-0.6675399214138267
1101	100	-0.7453747841699041
1102	1	-0.07514685137972421
1102	2	-0.11245895692299257
1102	3	-0.05183494770227526
1102	4	0.02155194838746155
1102	5	-0.0017236121846266883
1102	6	0.014790771811185266
1102	7	0.14519012574254475
1102	8	-0.04729166690341913
1102	9	-3.929143369774124E-4
1102	10-11	-0.03935054826290241
1102	12-13	-0.05299733459101219
1102	14-15	-0.04758652922740936
1102	16-17	-0.09867776002395345
1102	18-19	-0.12833824430159524
1102	20-21	-0.1190196795490408
1102	22-23	-0.15773234871689112
1102	24-25	-0.02062363789014654
1102	26-27	-0.1752113676277247
1102	28-29	-0.3952658781528058
1102	30-31	-0.15161837870547146
1102	32-33	-0.1558654978989793
1102	34-35	-0.15413238653362527
1102	36-37	-0.20141509205071628
1102	38-39	-0.31499754867965635
1102	40-41	-0.6816553557553178
1102	42-43	-0.0574152278621014
1102	44-45	-0.18340145845326816
1102	46-47	-0.21146880781552824
1102	48-49	-0.593438321705932
1102	50-51	-0.407423416785754
1102	52-53	-0.4676443746542347
1102	54-55	-0.1786839405312577
1102	56-57	-0.13359530520916252
1102	58-59	-0.16665369953075526
1102	60-61	-0.7156527547004146
1102	62-63	-0.2617678477566514
1102	64-65	-0.27027700051381487
1102	66-67	-0.2538066030621948
1102	68-69	-0.34648603888353335
1102	70-71	-0.18448977381870435
1102	72-73	-0.4048568454580561
1102	74-75	-0.2988086931816767
1102	76-77	-0.44644639945956754
1102	78-79	-0.3376728758680727
1102	80-81	-0.2881322193964664
1102	82-83	-0.30996031115331135
1102	84-85	-0.3484760580126398
1102	86-87	-0.4530658991414924
1102	88-89	-0.36727444588735025
1102	90-91	-0.3763164825627463
1102	92-93	-0.45026253791128923
1102	94-95	-0.6474212848493366
1102	96-97	-0.5979194686888896
1102	98-99	-0.7388620905706134
1102	100	-0.9162422252982374
1103	1	-0.09209563554061972
1103	2	-0.1479844577866274
1103	3	-0.1811511334076492
1103	4	-0.10180754538892245
1103	5	-0.05346844404217421
1103	6	-0.08491690804994789
1103	7	0.09982859287422485
1103	8	-0.12738907341989147
1103	9	-0.16666602476408343
1103	10-11	-0.116314952529045
1103	12-13	-0.08399154997889724
1103	14-15	-0.1582082546814334
1103	16-17	-0.11178438628240173
1103	18-19	-0.2513347179227381
1103	20-21	-0.25378736461208007
1103	22-23	-0.3358870495494841
1103	24-25	-0.31295320702240303
1103	26-27	-0.4038398807144077
1103	28-29	-0.4280489692092502
1103	30-31	-0.345584035310317
1103	32-33	-0.4849407538991386
1103	34-35	-0.42110012102920535
1103	36-37	-0.5462349327457616
1103	38-39	-0.41340798382964294
1103	40-41	-0.40680257560649835
1103	42-43	-0.30580687433903364
1103	44-45	-0.35086601504058734
1103	46-47	-0.375628641584278
1103	48-49	-0.5623893209903059
1103	50-51	-0.5212763090199601
1103	52-53	-0.4467221274283446
1103	54-55	-0.3990283731923654
1103	56-57	-0.334697424341563
1103	58-59	-0.23199310631163428
1103	60-61	-0.10491571062212302
1103	62-63	-0.25494752351372796
1103	64-65	-0.17731267600074574
1103	66-67	-0.22721739746022251
1103	68-69	-0.18260067062553986
1103	70-71	-0.30401977318642537
1103	72-73	-0.255685289425422
1103	74-75	-0.2043129840403921
1103	76-77	-0.4512518810100232
1103	78-79	-0.4037958026475721
1103	80-81	-0.4281169944640766
1103	82-83	-0.3631863227239691
1103	84-85	-0.4218451077871741
1103	86-87	-0.5533944494462801
1103	88-89	-0.4222006886700562
1103	90-91	-0.4326571747331478
1103	92-93	-0.5334974372787258
1103	94-95	-0.6659046868708955
1103	96-97	-0.6001887488132773
1103	98-99	-0.558323060425387
1103	100	-0.6413466946313875
1104	1	-0.050923615723192484
1104	2	-0.051971605809796984
1104	3	-0.01590491357821122
1104	4	-0.09489203723354223
1104	5	-0.03486542423880934
1104	6	-0.10630422112758708
1104	7	0.07213531126186012
1104	8	-0.054083609845100966
1104	9	-0.0937855147002793
1104	10-11	-0.1381651540819675
1104	12-13	-0.06787592732033687
1104	14-15	-0.06229755577850682
1104	16-17	-0.10999549418465193
1104	18-19	-0.20322802281005892
1104	20-21	-0.17364984980336118
1104	22-23	-0.11799053953139094
1104	24-25	-0.10071677797687073
1104	26-27	-0.166097568698234
1104	28-29	-0.3287708024675453
1104	30-31	-0.31842828343901175
1104	32-33	-0.23298036607008754
1104	34-35	-0.3589905094396215
1104	36-37	-0.3489834494067452
1104	38-39	-0.38253850646164267
1104	40-41	-0.12454420243837916
1104	42-43	-0.2648590240515176
1104	44-45	-0.24483916183669407
1104	46-47	-0.3591442562606275
1104	48-49	-0.30886731296701697
1104	50-51	-0.34196845287008415
1104	52-53	-0.28727932232725806
1104	54-55	-0.2628140323915673
1104	56-57	-0.8108958707506773
1104	58-59	-0.2085968988210425
1104	60-61	-0.18560745474727014
1104	62-63	-0.2292655322068029
1104	64-65	-0.1663169424709281
1104	66-67	-0.2371568393323642
1104	68-69	-0.3023978544361867
1104	70-71	-0.3247994305930533
1104	72-73	-0.22761816667502188
1104	74-75	-0.23242480963941148
1104	76-77	-0.3564276681533656
1104	78-79	-0.2604704017661206
1104	80-81	-0.29422916765179963
1104	82-83	-0.2158043789437727
1104	84-85	-0.389922236357954
1104	86-87	-0.3862634711032449
1104	88-89	-0.45611779246935313
1104	90-91	-0.6352489283538212
1104	92-93	-0.7053830437744395
1104	94-95	-0.44477037389603424
1104	96-97	-0.47783825009801717
1104	98-99	-0.4886088144261649
1104	100	-0.6393286364416291
1105	1	-0.05299816650441258
1105	2	-0.03176809475562692
1105	3	0.061836561823838565
1105	4	0.08037896997786476
1105	5	-0.022030845410917266
1105	6	0.07098129742355042
1105	7	0.1647655706125022
1105	8	0.07288850344493625
1105	9	0.0037314439480908845
1105	10-11	-0.013831897898654688
1105	12-13	-0.03018658876441549
1105	14-15	2.5768170263518186E-4
1105	16-17	-0.01955134384365209
1105	18-19	-0.06074902482075828
1105	20-21	-0.009851831213005369
1105	22-23	-0.05331612174303757
1105	24-25	-0.022103307100493907
1105	26-27	-0.04965287997655565
1105	28-29	-0.08760935537028303
1105	30-31	-0.05842396990960452
1105	32-33	-0.10027329114824113
1105	34-35	-0.1814455681304068
1105	36-37	-0.17514232233113347
1105	38-39	-0.09254209157899851
1105	40-41	-0.1715988106276214
1105	42-43	-0.1780338554760803
1105	44-45	-0.15881224350952294
1105	46-47	-0.116608344455301
1105	48-49	-0.07367670415814587
1105	50-51	-0.17526933844249726
1105	52-53	-0.0409775929398819
1105	54-55	-0.0391486375815262
1105	56-57	-0.056614558763570244
1105	58-59	-0.08693530426450025
1105	60-61	-0.03283085086862059
1105	62-63	-0.07487802584722658
1105	64-65	-0.08155720134170963
1105	66-67	-0.04395567326623251
1105	68-69	-0.07322210037020227
1105	70-71	-0.09333112168175006
1105	72-73	-0.06334148472846124
1105	74-75	-0.11102925693479193
1105	76-77	-0.051172870344238675
1105	78-79	-0.06615398592916932
1105	80-81	-0.026331506781382075
1105	82-83	-0.05528603302892776
1105	84-85	-0.08357801051771574
1105	86-87	-0.12598588355658435
1105	88-89	-0.05607807998449843
1105	90-91	-0.1519649666717413
1105	92-93	-0.23425564542059973
1105	94-95	-0.1757960244121648
1105	96-97	-0.09830142498243433
1105	98-99	-0.16380437228058753
1105	100	-0.19644471408756914
1106	1	-0.050963894327132664
1106	2	-0.053586498227936374
1106	3	0.004074570672905509
1106	4	0.024437500685571933
1106	5	0.058628914641900565
1106	6	0.0343070277585511
1106	7	0.1391319410084293
1106	8	-0.05804631129054627
1106	9	-0.058350737055498314
1106	10-11	-0.07384169249822747
1106	12-13	-0.04049251437766799
1106	14-15	-0.04477665684596843
1106	16-17	-0.07166550953368045
1106	18-19	-0.09617579035526802
1106	20-21	-0.14035086134133934
1106	22-23	-0.1592936950054451
1106	24-25	-0.10494610230389867
1106	26-27	-0.11856256556823297
1106	28-29	-0.11875734094478219
1106	30-31	-0.16427258406417167
1106	32-33	-0.09547465225227825
1106	34-35	-0.028461752876793867
1106	36-37	-0.06569434318268463
1106	38-39	-0.07537502766345483
1106	40-41	-0.03670844010735408
1106	42-43	0.004966705543829164
1106	44-45	-0.08239999939582532
1106	46-47	-0.043370334391518384
1106	48-49	-0.14176103951164976
1106	50-51	-0.06257117557809266
1106	52-53	-0.05373006758172494
1106	54-55	0.015152655460539677
1106	56-57	-0.03529104283726525
1106	58-59	0.029513097386328013
1106	60-61	-0.06843527370402569
1106	62-63	-0.06594890199990999
1106	64-65	-0.04113793011160993
1106	66-67	-0.032883271349682275
1106	68-69	0.0031067962533981586
1106	70-71	-0.013846470633325225
1106	72-73	0.08889428406346411
1106	74-75	0.07891860762206448
1106	76-77	0.05651221629364045
1106	78-79	0.004484301317049244
1106	80-81	0.10411970134303061
1106	82-83	0.037207626896467616
1106	84-85	0.04396049605258057
1106	86-87	-0.03961365918183546
1106	88-89	0.02854776007924187
1106	90-91	0.08863914461869271
1106	92-93	-0.02979491284166258
1106	94-95	0.052168989117291176
1106	96-97	0.03728616307382637
1106	98-99	0.0027025558164837093
1106	100	0.039109013241859
1107	1	-0.11241018107647349
1107	2	-0.10709074791443385
1107	3	-0.056675046158638054
1107	4	-0.04592616101151492
1107	5	-0.012570750956534482
1107	6	-0.0619727548804363
1107	7	0.04508150210638462
1107	8	-0.07755306898874892
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2316	92-93	0.39875775844556927
2316	94-95	0.3712642517690057
2316	96-97	0.5188340444780621
2316	98-99	0.43864062597568676
2316	100	0.4766878537698567
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	16624.0
1	1317.0
2	940.0
3	765.0
4	723.0
5	710.0
6	744.0
7	1204.0
8	1869.0
9	2544.0
10	4006.0
11	7368.0
12	11945.0
13	15730.0
14	17937.0
15	19873.0
16	22282.0
17	26112.0
18	30935.0
19	37215.0
20	43893.0
21	52287.0
22	60700.0
23	67072.0
24	70652.0
25	73312.0
26	77698.0
27	86060.0
28	98502.0
29	117084.0
30	142078.0
31	178416.0
32	233431.0
33	323585.0
34	509505.0
35	915521.0
36	1131758.0
37	1217436.0
38	1259295.0
39	297462.0
40	310.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.379109891188506	17.661632880274407	12.668294936627738	23.29096229190935
2	15.91862448312166	21.104145105349513	38.306076850467036	24.67115356106179
3	21.382420240279476	24.64584140122516	29.173748579946146	24.79798977854922
4	13.134765146999715	15.613354929783101	37.829103686733255	33.42277623648392
5	13.545237717938004	37.93967856709778	33.853632487747554	14.66145122721666
6	31.342396177141257	35.76350603890645	17.522269843344134	15.37182794060816
7	27.868305214276894	32.32529599552447	21.671679780629844	18.134719009568787
8	26.28699032703411	32.39367991334964	20.14685218014587	21.17247757947038
9	26.387834985251562	16.84530216823005	20.726909258663834	36.039953587854555
10-11	25.76403831393207	24.820047059454907	27.827047279155142	21.588867347457878
12-13	27.322949625330423	22.813622632872736	27.216941459298255	22.64648628249859
14-15	23.646386093512923	24.560939755018715	26.654219015767723	25.13845513570064
16-17	22.741902339741365	27.42864477422639	26.664831827486545	23.16462105854569
18-19	22.45540858550364	27.838135485663713	27.573993345533005	22.13246258329965
20-21	23.930337222202887	25.750967255134082	27.058143778724286	23.260551743938745
22-23	23.11367382977889	25.6271540649521	27.541918068151983	23.717254037117026
24-25	23.987977098956556	25.60437910017358	27.245640115417952	23.162003685451914
26-27	23.395707771574507	24.979977553056777	28.701961937749388	22.922352737619327
28-29	22.489594600054215	27.48496244438023	27.415986871434928	22.609456084130628
30-31	23.573450203949818	25.641098214241413	27.26295476386205	23.522496817946717
32-33	23.591863485144163	25.897448369306186	27.550785079505914	22.959903066043736
34-35	24.316968819316916	25.703399726616304	27.38035759325345	22.59927386081333
36-37	23.739638857912873	25.36831132123935	28.26616362628004	22.625886194567737
38-39	23.229637627936274	26.45053391648072	27.808076645316344	22.511751810266663
40-41	23.385660070364196	26.464717608800324	27.283852709915035	22.86576961092044
42-43	22.801347986470372	26.676997800184267	26.376576246882593	24.145077966462768
44-45	23.242647198589374	25.655690482393418	27.017526916105833	24.08413540291138
46-47	24.01678455626259	26.293260934407947	26.90855069737173	22.78140381195773
48-49	23.596244386123946	26.16666703948042	27.047865930210385	23.18922264418525
50-51	22.199710521916067	26.563668124028805	28.240769216575124	22.995852137480004
52-53	23.287103288521177	27.201145161240703	26.306980846767146	23.204770703470974
54-55	22.539142810518886	25.93289240588016	27.20033626789342	24.327628515707534
56-57	23.53773713861242	25.714629519195427	26.894411748850573	23.85322159334158
58-59	24.605216851966787	25.806550839467786	26.504836138130795	23.083396170434632
60-61	23.576212081285615	26.177048479057554	27.98891979150867	22.25781964814816
62-63	22.882090617220804	26.379587082040924	27.581702051557905	23.156620249180367
64-65	22.583774886661452	27.319651052625105	26.79452697586026	23.302047084853182
66-67	23.22384919821914	26.045863961021915	26.316930320099317	24.413356520659626
68-69	22.53033704346231	26.306996954271572	26.919498198624446	24.243167803641676
70-71	23.674956084226828	25.979245003202045	26.667514578050934	23.6782843345202
72-73	23.217007023910664	25.61062571891072	27.695031518444317	23.477335738734297
74-75	22.306125645152495	27.633984067477602	26.675333955122138	23.384556332247765
76-77	22.78483039022234	25.91460207096755	26.54641865164042	24.754148887169688
78-79	22.903207696743937	26.031105806738392	27.13068260271627	23.935003893801397
80-81	23.68411373957166	25.891047287257823	26.652875254848148	23.771963718322368
82-83	23.26240874886493	25.567316727499588	27.610486263888795	23.559788259746682
84-85	22.79087757992158	26.482878506779645	27.143495611338263	23.582748301960514
86-87	22.619984038812508	26.83168050999728	26.74031690522447	23.808018545965744
88-89	22.160836073979194	26.538349303674842	26.343359242987496	24.95745537935847
90-91	23.097415516688653	25.411553630117567	26.620768929844846	24.870261923348938
92-93	23.657414941790137	26.112088872036725	26.69908097929701	23.53141520687613
94-95	23.11923903583554	25.741155750788835	26.930604511293755	24.209000702081866
96-97	21.752622260872144	25.827439228119182	28.098578184204886	24.32136032680379
98-99	22.55403116718285	26.373673090130094	26.50162167967502	24.570674063012042
100	22.378977577983587	24.662931225843106	26.75018186950239	26.207909326670915
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10384.0
1	8041.0
2	4377.0
3	2502.5
4	1698.0
5	1574.5
6	2068.0
7	3328.0
8	5276.0
9	6467.0
10	6192.5
11	5727.5
12	5835.5
13	6238.0
14	6772.5
15	7265.0
16	7570.0
17	7997.5
18	8559.0
19	9213.5
20	9829.0
21	10718.5
22	12237.5
23	14459.5
24	17147.0
25	20790.0
26	25929.0
27	32063.0
28	39373.5
29	48445.5
30	58317.5
31	67155.0
32	77755.0
33	89336.0
34	99982.0
35	111192.0
36	126294.0
37	140875.5
38	151275.0
39	162213.0
40	172712.0
41	182174.0
42	191794.0
43	202750.0
44	215104.0
45	229532.0
46	249431.5
47	314337.0
48	441098.0
49	442989.0
50	334558.0
51	289825.0
52	277069.0
53	269443.5
54	262056.5
55	247788.5
56	226964.0
57	206175.5
58	185283.5
59	161421.5
60	137308.0
61	115215.5
62	95068.5
63	74868.0
64	55871.5
65	41710.5
66	30948.0
67	23793.5
68	18236.5
69	13353.5
70	10274.5
71	7839.5
72	5953.0
73	4559.0
74	3475.0
75	2611.5
76	1857.5
77	1226.5
78	785.0
79	512.0
80	364.5
81	284.0
82	199.5
83	147.0
84	121.0
85	98.0
86	74.5
87	61.0
88	56.5
89	42.5
90	33.0
91	32.0
92	28.0
93	25.5
94	23.5
95	18.0
96	11.0
97	8.0
98	8.5
99	6.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.20478200894536638
2	0.18422996001058953
3	0.3712327049283117
4	0.36703869358636737
5	0.3776421574774624
6	0.3071660466218005
7	0.32505677938943
8	0.31533113182571865
9	0.3288327829564297
10-11	0.3023032228399448
12-13	0.31019660299014895
14-15	0.297245328763115
16-17	0.29204113196505455
18-19	0.31501065919826104
20-21	0.3189817330602349
22-23	0.31014086862015633
24-25	0.3142512784071117
26-27	0.3038289512184927
28-29	0.3294319274338503
30-31	0.31101868494754004
32-33	0.3112485892237596
34-35	0.3133177277097354
36-37	0.32141314495116274
38-39	0.3279131658515515
40-41	0.35382268110186854
42-43	0.32213072496481765
44-45	0.30759798798924326
46-47	0.31303208906352326
48-49	0.3356323760955287
50-51	0.32515431453691707
52-53	0.3541640541180733
54-55	0.35473533141049757
56-57	0.3599673953935543
58-59	0.3456784962866976
60-61	0.32890245091892045
62-63	0.3513076676559517
64-65	0.3466120469840739
66-67	0.3323788822472098
68-69	0.34259917234460563
70-71	0.36221070378575704
72-73	0.36680182251389876
74-75	0.3551672727779403
76-77	0.3392760105337959
78-79	0.35040895093982083
80-81	0.363290557204364
82-83	0.3684181192436846
84-85	0.36030876840975906
86-87	0.35952848722986247
88-89	0.34829801167635055
90-91	0.36028786802101187
92-93	0.3414287505747607
94-95	0.3626147779682035
96-97	0.3717064470732489
98-99	0.36200866669453385
100	0.3837729381766501
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7176900.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.52674278078635
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.14654139881435	40.429693909380774
2	14.540831068744147	15.857283110085579
3	4.932260022566162	8.068202207352618
4	2.228321752130737	4.860125080450555
5	1.1654652129414156	3.177451094300548
6	0.7141046086802675	2.3362678989649823
7	0.46965218299258316	1.792602264492997
8	0.32533749943699447	1.4191675318996178
9	0.2480585665661797	1.2173243088352186
>10	1.1451540751219893	10.893215342948032
>50	0.05814218492477145	2.1626445943501973
>100	0.023961307275551087	2.2578164643700007
>500	0.0012308194755742284	0.4552165693851613
>1k	5.506243309652318E-4	0.5722703600788667
>5k	6.477933304684885E-5	0.30415826129121337
>10k+	3.238966652342442E-4	4.196561001813624
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	86832	1.2098817037996907	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	25365	0.3534255737156711	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20653	0.2877704858643704	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	19250	0.26822165558946065	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	17799	0.24800401287463947	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16302	0.22714542490490322	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	14085	0.19625465033649625	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	14018	0.19532109963911995	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	12320	0.1716618595772548	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	10993	0.15317198233220472	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9353	0.13032089063523247	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	7869	0.10964343936797223	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.78671849963076E-5
2	0.0	0.0	0.0	0.0	2.78671849963076E-5
3	0.0	0.0	0.0	0.0	2.78671849963076E-5
4	0.0	0.0	0.0	1.39335924981538E-5	2.78671849963076E-5
5	0.0	1.39335924981538E-5	0.0	1.39335924981538E-5	2.78671849963076E-5
6	0.0	1.39335924981538E-5	0.0	1.39335924981538E-5	5.57343699926152E-5
7	0.0	1.39335924981538E-5	0.0	1.39335924981538E-5	6.966796249076899E-5
8	0.0	1.39335924981538E-5	0.0	1.39335924981538E-5	6.966796249076899E-5
9	0.0	1.39335924981538E-5	0.0	6.966796249076899E-5	1.114687399852304E-4
10-11	1.39335924981538E-5	1.39335924981538E-5	0.0	9.05683512379997E-5	1.114687399852304E-4
12-13	1.39335924981538E-5	1.39335924981538E-5	0.0	1.045019437361535E-4	1.6023631372876867E-4
14-15	1.39335924981538E-5	1.39335924981538E-5	0.0	1.114687399852304E-4	2.020370912232301E-4
16-17	1.39335924981538E-5	1.39335924981538E-5	0.0	1.532695174796918E-4	2.368710724686146E-4
18-19	1.39335924981538E-5	1.39335924981538E-5	0.0	1.8810349872507628E-4	2.647382574649222E-4
20-21	1.39335924981538E-5	1.39335924981538E-5	0.0	3.5530660870292184E-4	2.7867184996307597E-4
22-23	1.39335924981538E-5	1.39335924981538E-5	0.0	5.852108849224596E-4	3.1350583120846046E-4
24-25	1.39335924981538E-5	1.39335924981538E-5	0.0	8.847831236327662E-4	3.4833981245384495E-4
26-27	1.39335924981538E-5	1.39335924981538E-5	0.0	0.0016023631372876867	3.5530660870292184E-4
28-29	1.39335924981538E-5	1.39335924981538E-5	0.0	0.004061642213211833	3.622734049519988E-4
30-31	1.39335924981538E-5	1.39335924981538E-5	0.0	0.011815686438434421	3.7620699745015255E-4
32-33	1.39335924981538E-5	1.39335924981538E-5	0.0	0.03084897379091251	4.0407418244646016E-4
34-35	1.39335924981538E-5	1.39335924981538E-5	0.0	0.06066686173696164	4.5980855243907536E-4
36-37	1.39335924981538E-5	1.39335924981538E-5	0.0	0.10524739093480472	4.5980855243907536E-4
38-39	1.39335924981538E-5	1.39335924981538E-5	0.0	0.16947428555504465	4.807089411863061E-4
40-41	2.78671849963076E-5	1.39335924981538E-5	0.0	0.24107205060680797	4.8767573743538296E-4
42-43	2.78671849963076E-5	1.39335924981538E-5	0.0	0.3127882511948056	4.946425336844599E-4
44-45	2.78671849963076E-5	1.39335924981538E-5	0.0	0.4048056960526133	5.085761261826136E-4
46-47	2.78671849963076E-5	1.39335924981538E-5	0.0	0.5156056235979323	5.155429224316905E-4
48-49	2.78671849963076E-5	1.39335924981538E-5	0.0	0.6283283869079965	6.827460324095361E-4
50-51	2.78671849963076E-5	1.39335924981538E-5	0.0	0.7497457119369086	6.966796249076899E-4
52-53	2.78671849963076E-5	1.39335924981538E-5	0.0	0.8766459056138445	7.524139949003051E-4
54-55	2.78671849963076E-5	1.39335924981538E-5	0.0	1.0232203318981732	8.36015549889228E-4
56-57	2.78671849963076E-5	1.39335924981538E-5	0.0	1.2045521046691468	8.778163273836894E-4
58-59	2.78671849963076E-5	1.39335924981538E-5	0.0	1.3968774819211638	9.196171048781507E-4
60-61	2.78671849963076E-5	1.39335924981538E-5	0.0	1.5981412587607462	9.474842898744584E-4
62-63	2.78671849963076E-5	1.39335924981538E-5	0.0	1.8108514818375623	0.0010171522523652274
64-65	2.78671849963076E-5	2.09003887472307E-5	0.0	2.031253047973359	0.0010171522523652274
66-67	2.78671849963076E-5	2.78671849963076E-5	0.0	2.2905641711602502	0.0010310858448633812
68-69	2.78671849963076E-5	2.78671849963076E-5	0.0	2.5951803703548886	0.001045019437361535
70-71	2.78671849963076E-5	2.78671849963076E-5	0.0	2.914587077986317	0.0010868202148559963
72-73	2.78671849963076E-5	2.78671849963076E-5	0.0	3.2263442433362592	0.0012261561398375343
74-75	2.78671849963076E-5	2.78671849963076E-5	0.0	3.5596775766695927	0.0012261561398375343
76-77	2.78671849963076E-5	2.78671849963076E-5	0.0	3.915569396257437	0.0012540233248338418
78-79	2.78671849963076E-5	2.78671849963076E-5	0.0	4.349572935389932	0.0012540233248338418
80-81	2.78671849963076E-5	2.78671849963076E-5	0.0	4.802310189636193	0.0013097576948264572
82-83	2.78671849963076E-5	2.78671849963076E-5	0.0	5.241406456826764	0.001372458861068149
84-85	2.78671849963076E-5	2.78671849963076E-5	0.0	5.700810935083393	0.0013794256573172261
86-87	3.4833981245384496E-5	2.78671849963076E-5	0.0	6.183463612423191	0.0014003260460644569
88	4.18007774944614E-5	2.78671849963076E-5	0.0	6.572015772826708	0.0014212264348116876
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	27805	0.0	27.596497	1
TACATGG	28415	0.0	26.637245	2
ACATGGG	29050	0.0	25.425797	3
CATGGGG	20505	0.0	24.37129	4
TACCTGG	4760	0.0	22.913538	2
ATGGGGG	12515	0.0	22.464336	5
GAGTACT	18410	0.0	20.10942	12-13
AGAGTAC	31810	0.0	18.35781	10-11
GTACTTT	19930	0.0	18.351852	14-15
AGTACTT	19215	0.0	18.227802	12-13
GTACCTG	6585	0.0	17.350307	1
TGGGGGG	11990	0.0	17.203005	6
GAGTACA	21605	0.0	16.974503	1
AGTACAT	21045	0.0	16.240396	2
GTATAGG	1845	0.0	16.054651	1
CATGGGA	9345	0.0	15.896912	4
GTGTAGC	3395	0.0	15.510826	1
GTATAAT	2935	0.0	15.378707	1
ACTTTTT	25040	0.0	14.962903	16-17
TACTTTT	23095	0.0	14.840071	14-15
>>END_MODULE