FastQCFastQC Report
Mon 6 Jun 2016
ERR523191_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523191_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7176900
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT889391.2392397831933009No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT252920.3524084214633059No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC198510.2765957446808511No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194990.27169112012150093No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC185680.2587189455057197No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161040.22438657359026876No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG146090.20355585280552885No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA144810.20177235296576515No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA132610.1847733701180175No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC114050.15891262244144408No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90930.12669815658571248No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG279750.026.6147021
TACATGG287700.025.5685882
ACATGGG292000.024.4466533
CATGGGG202750.023.1552314
TACCTGG50600.021.9226862
ATGGGGG118950.021.6103275
GAGTACT185300.019.44569612-13
GGGTTAG23350.018.7329791
GTATAGG18100.018.1898981
GTACTTT196150.018.09167714-15
GTACCTG65950.017.829371
AGAGTAC325000.017.75517310-11
TGGGGGG105050.017.7148536
GAGTACA221250.017.0703741
AGTACTT194700.016.91332212-13
AGTACAT212150.016.72772
CATGGGA100000.015.9780384
ACCTGGG70750.015.4765443
ACTTTTT239300.014.92514116-17
GGACCGT9250.014.7331216
Produced by FastQC (version 0.11.5)