FastQCFastQC Report
Mon 6 Jun 2016
ERR523190_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523190_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6459135
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216100.3345649223928591No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174320.26988133860029245No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101180.1566463620902799No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101400.051.7806051
GTATCAA182550.041.622831
TACCTGG65450.036.410892
TCAACGC224300.033.3569344
ATCAACG229000.032.7559283
CAACGCA229000.032.6507765
TATCAAC234700.032.063522
AACGCAG238000.031.6439536
GTACCTG80550.030.1387351
ACCTGGG83050.028.01293
ACGCAGA270950.027.6572557
GTACATG207050.027.1539841
CGCAGAG281350.026.6169788
TACATGG215450.025.7217372
ACATGGG222800.023.9637993
GCAGAGT309800.023.8835939
GTGGTAT55500.023.3120571
CATGGGG157950.022.3753854
TGGTATC55550.021.746132
GAGTACT185250.021.20201712-13
Produced by FastQC (version 0.11.5)