FastQCFastQC Report
Mon 6 Jun 2016
ERR523190_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523190_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6459135
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195380.30248632363311806No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168190.2603909037355621No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97120.15036069071168198No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104150.053.565011
GTATCAA181350.039.659361
TACCTGG68850.038.86512
GTACCTG84200.032.3461271
TCAACGC222300.032.006284
CAACGCA225750.031.5171475
ATCAACG226350.031.247473
ACCTGGG85100.031.1464423
TATCAAC232250.030.6361872
AACGCAG237100.030.1072886
ACGCAGA270900.026.3506187
GTACATG213700.025.885621
CGCAGAG282100.025.244068
TACATGG220300.024.5490572
ACATGGG224900.023.278483
GCAGAGT315700.022.4531069
CATGGGG159850.021.5496464
GAGTACT188750.020.69571912-13
CAGAGTA305000.020.34419810-11
GTGGTAT56050.020.2254051
Produced by FastQC (version 0.11.5)