FastQCFastQC Report
Mon 6 Jun 2016
ERR523189_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523189_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12649667
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT332340.26272628362469935No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258230.2041397611494437No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156630.12382144130750636No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA211550.043.971821
GTATCAA358300.037.3082851
TCAACGC423350.030.9500144
CAACGCA431150.030.3890115
ATCAACG431650.030.3454463
AACGCAG445150.029.4906566
TATCAAC450700.029.233722
GTACATG445450.027.6430261
TACATGG463850.026.084322
ACGCAGA508200.025.6933087
ACATGGG463200.025.3258673
CGCAGAG526950.024.742248
CATGGGG308000.022.7964654
GCAGAGT582600.022.1193969
GAGTACT306950.020.1129112-13
AGAGTAC505700.019.49064310-11
CAGAGTA569800.019.09544410-11
ATGGGGG173950.018.911495
GTACTTT326150.018.90015614-15
GTATAGG39200.018.484831
Produced by FastQC (version 0.11.5)