FastQCFastQC Report
Mon 6 Jun 2016
ERR523189_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523189_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12649667
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT301940.23869403044364726No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256720.20294605383683223No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151550.11980552531540949No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA213850.045.4044881
GTATCAA358150.037.0672531
TCAACGC430600.030.2417834
CAACGCA437550.029.7506875
ATCAACG439550.029.6055623
AACGCAG455050.028.6266426
TATCAAC460150.028.4654182
GTACATG448900.027.4358461
TACATGG466450.025.9635432
ACGCAGA518950.025.0653327
ACATGGG475950.024.6259923
CGCAGAG535850.024.210298
CATGGGG310250.022.2967154
GCAGAGT603650.021.3507429
GAGTACT312350.020.70699512-13
AGAGTAC515100.019.82025710-11
GTACTTT329900.019.31817214-15
ATGGGGG170100.019.3115775
CAGAGTA587300.018.82828110-11
AGTACTT342450.017.74081412-13
Produced by FastQC (version 0.11.5)