##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR523189_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12649667
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.69519592887307	34.0	31.0	34.0	30.0	35.0
2	32.756238168166796	34.0	31.0	34.0	31.0	34.0
3	33.03550963041162	34.0	31.0	34.0	31.0	36.0
4	35.81642900164882	37.0	35.0	37.0	33.0	37.0
5	35.86855440542427	37.0	35.0	37.0	35.0	37.0
6	35.94220187772532	37.0	35.0	37.0	35.0	37.0
7	35.76560110238475	37.0	35.0	37.0	35.0	37.0
8	35.81861332792397	37.0	36.0	37.0	35.0	37.0
9	37.04320951689875	39.0	36.0	39.0	34.0	39.0
10-11	37.1675938583996	39.0	36.5	39.0	34.0	39.0
12-13	37.03685836156794	39.0	36.5	39.0	34.0	39.0
14-15	38.14545659581395	39.0	36.5	41.0	34.5	41.0
16-17	38.16849763713147	39.0	37.0	41.0	34.5	41.0
18-19	38.17408351540005	39.0	37.0	41.0	34.0	41.0
20-21	38.06295335679587	39.0	36.5	41.0	34.0	41.0
22-23	37.98029647736972	39.0	36.5	41.0	34.5	41.0
24-25	37.853995919418274	39.0	36.0	41.0	34.0	41.0
26-27	37.73317420134459	39.0	36.5	41.0	34.0	41.0
28-29	37.70267019677277	39.0	36.5	41.0	34.5	41.0
30-31	37.55847331791422	38.5	36.0	41.0	34.0	41.0
32-33	37.43249620721241	38.0	36.5	41.0	33.0	41.0
34-35	37.26484365161549	38.0	36.0	41.0	33.0	41.0
36-37	37.28942785608507	38.0	36.0	41.0	33.0	41.0
38-39	37.43835912834702	38.0	36.5	41.0	33.0	41.0
40-41	37.34279704754283	38.0	36.5	41.0	33.0	41.0
42-43	37.378417115644226	38.0	36.5	41.0	33.5	41.0
44-45	37.2256842413322	38.0	36.0	41.0	33.0	41.0
46-47	36.97427983677357	38.0	36.5	41.0	32.5	41.0
48-49	36.951588923249915	38.0	36.5	41.0	32.5	41.0
50-51	36.57026837149152	38.0	35.5	40.5	31.0	41.0
52-53	36.63097289438528	38.0	36.0	40.0	31.0	41.0
54-55	36.43821849223383	37.5	36.0	40.0	31.0	41.0
56-57	36.14789503945045	38.0	35.5	40.0	30.0	41.0
58-59	36.02029017838968	37.0	35.0	40.0	30.5	41.0
60-61	35.75573677156877	36.5	35.0	40.0	30.0	41.0
62-63	35.58330092800071	37.5	35.0	40.0	29.0	41.0
64-65	35.44476281470493	37.0	35.0	39.0	29.0	41.0
66-67	35.22420598107444	36.5	34.5	39.0	29.0	41.0
68-69	34.75772634963434	36.0	34.0	38.5	28.0	40.5
70-71	34.5200242425354	36.0	34.0	38.0	28.0	40.0
72-73	34.257378237703804	36.5	34.0	37.0	27.0	39.5
74-75	33.97837049781627	35.5	34.0	37.5	26.5	39.0
76-77	32.48651438808626	34.5	31.5	36.5	25.0	37.5
78-79	33.489616446029764	35.0	33.0	37.0	26.5	38.0
80-81	33.47901932912542	35.0	34.0	36.5	27.0	37.0
82-83	33.29796215188905	35.0	33.5	36.5	26.5	37.5
84-85	33.09432485455942	35.0	33.0	36.5	26.0	37.0
86-87	32.8478853633064	35.0	33.0	36.0	26.0	37.0
88-89	32.68440947892146	35.0	33.0	36.0	25.5	36.5
90-91	32.54602563846147	35.0	33.0	35.5	25.0	37.0
92-93	32.39634956398457	35.0	32.5	35.5	25.0	36.5
94-95	32.27782276798275	35.0	33.0	35.0	25.0	36.0
96-97	32.10791703054318	35.0	32.0	35.0	24.0	36.5
98-99	31.986164141712187	35.0	32.0	35.0	24.0	36.5
100	30.800083037759016	34.0	30.0	35.0	19.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.07067309363743135
1101	2	-0.07848069757734066
1101	3	-0.12029804195945104
1101	4	-0.11672426219098497
1101	5	-0.07069352493374481
1101	6	-0.1618865867837158
1101	7	-0.11604278388380607
1101	8	-0.09121577189937824
1101	9	-0.15852408422620812
1101	10-11	-0.11520511040696846
1101	12-13	-0.1706444471134816
1101	14-15	-0.24650761402454435
1101	16-17	-0.12953857399489976
1101	18-19	-0.1118202968632005
1101	20-21	-0.17322614683818216
1101	22-23	-0.1105605069504847
1101	24-25	-0.23492708123905004
1101	26-27	-0.2277315069183743
1101	28-29	-0.23225100400176046
1101	30-31	-0.25028376976613487
1101	32-33	-0.22802814117416403
1101	34-35	-0.17393518798314034
1101	36-37	-0.3309861595907151
1101	38-39	-0.2052203255399192
1101	40-41	-0.2747348121195401
1101	42-43	-0.23369279178740499
1101	44-45	-0.3741826650947502
1101	46-47	-0.3811430818455932
1101	48-49	-0.45736464013435807
1101	50-51	-0.3351472596022802
1101	52-53	-0.49099624314538204
1101	54-55	-0.42017390081390715
1101	56-57	-0.49693786489282843
1101	58-59	-0.5791846634039004
1101	60-61	-0.6047136280280014
1101	62-63	-0.4208237246604085
1101	64-65	-0.46429880481753827
1101	66-67	-0.5393387317926823
1101	68-69	-0.47465916455598034
1101	70-71	-0.529322337194067
1101	72-73	-0.6786390196519889
1101	74-75	-0.6214639388367189
1101	76-77	-0.5023574295222204
1101	78-79	-0.47670894023057286
1101	80-81	-0.3431656401904206
1101	82-83	-0.47094516081449456
1101	84-85	-0.4855810449741895
1101	86-87	-0.5765840532984114
1101	88-89	-0.5240652324423678
1101	90-91	-0.5593654142851037
1101	92-93	-0.794334104386067
1101	94-95	-0.7242490873218159
1101	96-97	-0.5950277947638405
1101	98-99	-0.6204031002525277
1101	100	-0.679963157658733
1102	1	-0.025098308150198534
1102	2	-0.05998566782642456
1102	3	-0.06717099565979368
1102	4	-0.05701813776452269
1102	5	-0.07328619912135537
1102	6	-0.10595270163494774
1102	7	-0.12423458218601269
1102	8	-0.04783108139191938
1102	9	-0.09688313429492013
1102	10-11	-0.10150812822509891
1102	12-13	-0.10615918714700001
1102	14-15	-0.18411224294154493
1102	16-17	-0.09458782824295753
1102	18-19	-0.10876124994172187
1102	20-21	-0.17269844114483135
1102	22-23	-0.10781240697519223
1102	24-25	-0.1616517714985477
1102	26-27	-0.16294258633458014
1102	28-29	-0.1561142651680072
1102	30-31	-0.16342697187878485
1102	32-33	-0.21046476012681836
1102	34-35	-0.16385068945709946
1102	36-37	-0.2103564548300909
1102	38-39	-0.25014131560323705
1102	40-41	-0.24845746811993763
1102	42-43	-0.2505390225904591
1102	44-45	-0.30984359902281966
1102	46-47	-0.2523842278592383
1102	48-49	-0.1616790623923734
1102	50-51	-0.10580931413720407
1102	52-53	-0.17128407221039055
1102	54-55	-0.23680892345016957
1102	56-57	-0.10574792346002937
1102	58-59	-0.3067654305475216
1102	60-61	-0.2246360629588935
1102	62-63	-0.13183165092733162
1102	64-65	-0.2173774122894585
1102	66-67	-0.30142474862181245
1102	68-69	-0.25245093005063524
1102	70-71	-0.20069842334822852
1102	72-73	-0.3447115071798734
1102	74-75	-0.6980670122560255
1102	76-77	-0.30331599186467884
1102	78-79	-0.32370798366147824
1102	80-81	-0.21450250987290076
1102	82-83	-0.2451192354077989
1102	84-85	-0.26939521879128137
1102	86-87	-0.274152374553033
1102	88-89	-0.2006394943439318
1102	90-91	-0.23971367028702417
1102	92-93	-0.3575583430648379
1102	94-95	-0.46428009236788625
1102	96-97	-0.505780251277141
1102	98-99	-0.5960200300949019
1102	100	-0.3605211912654944
1103	1	-0.05868979985696399
1103	2	-0.07349538389414079
1103	3	-0.0927958174074277
1103	4	-0.009955451312571029
1103	5	-0.11993782300567801
1103	6	-0.07671303373966509
1103	7	-0.04829235915343588
1103	8	-0.025648171633093853
1103	9	-0.050845642865589014
1103	10-11	-0.04373504475776002
1103	12-13	-0.04511742533519225
1103	14-15	-0.10916873854153408
1103	16-17	-0.05627642632643415
1103	18-19	-0.02563429487463509
1103	20-21	-0.0903251600582351
1103	22-23	0.01587344830776516
1103	24-25	-0.08152199245164837
1103	26-27	-0.11070801925762908
1103	28-29	-0.07578711581719233
1103	30-31	-0.07518625509168686
1103	32-33	-0.005404651234265145
1103	34-35	0.017689774861850083
1103	36-37	-0.010429826905991035
1103	38-39	-0.08001531063518286
1103	40-41	-0.1509144134760163
1103	42-43	-0.1778826702418712
1103	44-45	-0.26249374341132636
1103	46-47	-0.21617721696730996
1103	48-49	-0.23133777077467954
1103	50-51	-0.05759921450156469
1103	52-53	-0.14307913607914458
1103	54-55	-0.152246319122753
1103	56-57	-0.02365386580828499
1103	58-59	-0.12730439700845153
1103	60-61	-0.14516025729751192
1103	62-63	-0.13453272001757455
1103	64-65	-0.24314396555031692
1103	66-67	-0.2135840602334227
1103	68-69	-0.16782530274316088
1103	70-71	-0.30977532910970496
1103	72-73	-0.3024135882503316
1103	74-75	-0.19756791966901943
1103	76-77	-0.1831869761314593
1103	78-79	-0.20565372860610864
1103	80-81	-0.17403265043998317
1103	82-83	-0.2020713111951551
1103	84-85	-0.1926162987820348
1103	86-87	-0.20300889192935756
1103	88-89	-0.24197494174541134
1103	90-91	-0.19107933072171335
1103	92-93	-0.22182569287290477
1103	94-95	0.010750566056415778
1103	96-97	-0.7665132922040634
1103	98-99	-1.053692274922355
1103	100	-0.3642723594695241
1104	1	-0.06553625868279056
1104	2	-0.0812508914348129
1104	3	-0.10004838956463402
1104	4	-0.11919833332506613
1104	5	-0.17813499814366196
1104	6	-0.12248975969073683
1104	7	-0.15050981259414442
1104	8	-0.045704960837582576
1104	9	-0.0890659793963735
1104	10-11	-0.15552878712207274
1104	12-13	-0.13639869629264467
1104	14-15	-0.24918301629883643
1104	16-17	-0.0939254022709477
1104	18-19	-0.1658714905514742
1104	20-21	-0.2665028456511678
1104	22-23	-0.15969081977606692
1104	24-25	-0.1690589333227095
1104	26-27	-0.10146397877602453
1104	28-29	-0.20836155326247052
1104	30-31	-0.2737418656911714
1104	32-33	-0.2040819541454013
1104	34-35	-0.14425214793572394
1104	36-37	-0.27181723810177516
1104	38-39	-0.30775643767932337
1104	40-41	-0.235631976958814
1104	42-43	-0.2620118456398117
1104	44-45	-0.3141940695977823
1104	46-47	-0.2190790385326551
1104	48-49	-0.28411230866721127
1104	50-51	-0.16161374003143436
1104	52-53	-0.21529074773962265
1104	54-55	-0.28048278284186523
1104	56-57	-0.3158221508488168
1104	58-59	-0.29920010050867063
1104	60-61	-0.3395703180125267
1104	62-63	-0.21761714477735694
1104	64-65	-0.3361530001862718
1104	66-67	-0.43881076627206284
1104	68-69	-0.2896708743663936
1104	70-71	-0.27663594385384016
1104	72-73	-0.3287133556402253
1104	74-75	-0.39529870775083253
1104	76-77	-0.3894838318690219
1104	78-79	-0.4253622611480665
1104	80-81	-0.24061101879065916
1104	82-83	-0.2400734891420484
1104	84-85	-0.23620614276796204
1104	86-87	-0.2964550863695621
1104	88-89	-0.2557630086596845
1104	90-91	-0.3110610279084298
1104	92-93	-0.3452696616292954
1104	94-95	-0.3967274900044515
1104	96-97	-0.16590047140642383
1104	98-99	-1.0782106370198044
1104	100	-0.4275005737151041
1105	1	-0.06835547414046061
1105	2	-0.9704345137993222
1105	3	-0.12797326670594344
1105	4	-0.038914666814747534
1105	5	-0.06886619968123142
1105	6	-0.05504900195725071
1105	7	-0.060210701366465
1105	8	-0.10237531836406788
1105	9	-0.13974598250683812
1105	10-11	-0.04203171495884561
1105	12-13	-0.016824836240573404
1105	14-15	-0.06093157562305862
1105	16-17	-0.5430694689897777
1105	18-19	-0.043086840145917904
1105	20-21	-0.13287377867995076
1105	22-23	-0.11952012273056312
1105	24-25	-0.1242227677336345
1105	26-27	-0.10737668517486298
1105	28-29	-0.17312625488565203
1105	30-31	-0.13910675351801416
1105	32-33	-0.09111243880494868
1105	34-35	-0.08973063328324571
1105	36-37	-0.10829940043092279
1105	38-39	-0.17630270258359104
1105	40-41	-0.1414032533113314
1105	42-43	-0.17988958726409265
1105	44-45	-0.34883044748287517
1105	46-47	-0.2677789729935398
1105	48-49	-0.2248194073608616
1105	50-51	-0.17442213226248526
1105	52-53	-0.2951562745615277
1105	54-55	-0.2819721417918615
1105	56-57	-0.14227923035467427
1105	58-59	-0.2783187271753391
1105	60-61	-0.22387084390699385
1105	62-63	-0.11330278750614298
1105	64-65	-0.11856295665538141
1105	66-67	-0.16933047416494418
1105	68-69	-0.2237187303034105
1105	70-71	-0.30385212967712505
1105	72-73	-0.31488470105362154
1105	74-75	-0.24701382701601204
1105	76-77	-0.17402281814643317
1105	78-79	-0.15399304554640025
1105	80-81	-0.16842392968914055
1105	82-83	-0.11649936038514852
1105	84-85	-0.09367968294607465
1105	86-87	-0.15201441407794647
1105	88-89	-0.16290580769600638
1105	90-91	-0.12837328177356255
1105	92-93	-0.16710149873615876
1105	94-95	-0.20869459306467775
1105	96-97	-0.17697698568929
1105	98-99	-0.18139702010164527
1105	100	-0.201923104640791
1106	1	-0.08430617379227101
1106	2	-0.08885664824821049
1106	3	-0.09419384955881327
1106	4	-0.050303640470701794
1106	5	-0.06254735971819514
1106	6	-0.023162366124196865
1106	7	-0.0519981729063943
1106	8	-0.009599171499644399
1106	9	-0.06668917212532222
1106	10-11	-0.10698343592468262
1106	12-13	-0.04026641895222127
1106	14-15	-0.09139042354245674
1106	16-17	0.023764954860311605
1106	18-19	-0.001964926487957541
1106	20-21	-0.04097375928860458
1106	22-23	0.0166575394326145
1106	24-25	0.044131039683406925
1106	26-27	0.025489529298795333
1106	28-29	0.01734455492450593
1106	30-31	0.021412000538518328
1106	32-33	0.11356576326878809
1106	34-35	0.09745271326347194
1106	36-37	-0.006215997609032797
1106	38-39	-0.016424525324417516
1106	40-41	-0.03913901040223777
1106	42-43	-0.053259522405738835
1106	44-45	-0.03878779174031166
1106	46-47	-0.07442878933745334
1106	48-49	-0.13089582939748823
1106	50-51	-0.03576797868928594
1106	52-53	-0.10741824777343112
1106	54-55	-0.028765299753381157
1106	56-57	-0.01868153636900871
1106	58-59	-0.10452333814023973
1106	60-61	-0.05483824616398891
1106	62-63	-0.08928349080507303
1106	64-65	-0.0828133866725338
1106	66-67	-0.02581929411676498
1106	68-69	0.01734077662350586
1106	70-71	-0.015350669907903125
1106	72-73	-0.05078825229462325
1106	74-75	-0.030852993966561826
1106	76-77	-0.08400500455278603
1106	78-79	-0.05702655847544236
1106	80-81	0.05202514476303577
1106	82-83	-0.0029361537672940585
1106	84-85	0.09271657054241444
1106	86-87	0.03286141320456437
1106	88-89	0.0679059555192012
1106	90-91	0.06396831327080577
1106	92-93	0.006973625755435364
1106	94-95	-0.042271917189118824
1106	96-97	-0.08753278512102725
1106	98-99	-0.07264973090101634
1106	100	0.004025010658036621
1107	1	-0.021761735935825754
1107	2	-0.07207931395163314
1107	3	-0.08695364415195428
1107	4	-0.07502025764435416
1107	5	-0.06704968771368414
1107	6	-0.09526368709846622
1107	7	-0.03893971245374672
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2316	64-65	-0.0956280510814409
2316	66-67	-0.13753641136726458
2316	68-69	0.027879638591464584
2316	70-71	-0.03156731151582193
2316	72-73	0.02627215759312662
2316	74-75	-0.03913418257302226
2316	76-77	-0.1583636243551112
2316	78-79	-0.15674538856959686
2316	80-81	-0.08496903305908177
2316	82-83	-0.05921575769102816
2316	84-85	-0.025626290883359104
2316	86-87	-0.143980449830174
2316	88-89	-0.06991483288359746
2316	90-91	-0.09973886023676215
2316	92-93	-0.17552630183111972
2316	94-95	-0.12264931135639046
2316	96-97	-0.04621538423845806
2316	98-99	0.026557598532637172
2316	100	-0.12759608979811787
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	7.0
12	44.0
13	180.0
14	767.0
15	2268.0
16	4942.0
17	9616.0
18	16399.0
19	24646.0
20	35832.0
21	51024.0
22	67276.0
23	77820.0
24	84291.0
25	92102.0
26	107150.0
27	127452.0
28	155764.0
29	191656.0
30	239790.0
31	302364.0
32	388588.0
33	523076.0
34	791458.0
35	1530899.0
36	2758429.0
37	2104954.0
38	2415073.0
39	545444.0
40	353.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.11158687811957	19.228803539900547	13.631631550733253	24.027978031246633
2	15.669381262759902	20.596905225956796	40.00790367172626	23.725809839557048
3	18.620351295939578	25.68969549092051	29.99822598570448	25.69172722743543
4	12.894369264124943	16.94956884615327	37.95625827443123	32.19980361529056
5	13.509987039797796	37.76375560951292	34.88344276854078	13.842814582148502
6	31.17136316638559	37.196845384021074	17.774403738574573	13.85738771101876
7	27.441341138967623	32.479173758421766	22.302256834291118	17.777228268319494
8	25.620875020069793	35.62937567941834	20.393076243396337	18.35667305711553
9	26.466204952163157	15.84791501181375	20.359022974839487	37.3268570611836
10-11	24.68355583659946	26.191271713174686	28.623494322715043	20.501678127510814
12-13	25.286934612229466	23.828468419583483	28.594079097310054	22.290517870876993
14-15	22.736571793051827	25.387573708945798	26.029023629359017	25.846830868643362
16-17	22.385614152795316	28.318547621896396	26.733099103536816	22.562739121771468
18-19	22.262530329897743	27.494466841643884	28.465248872162203	21.777753956296173
20-21	23.005001289167275	26.61725471443314	28.506881836978977	21.870862159420604
22-23	22.873393622762013	26.392988108709435	28.236755565035125	22.496862703493424
24-25	22.628760169125197	26.65075650038215	28.30066712180752	22.419816208685138
26-27	22.500719538328553	26.80120079987442	28.367910768629795	22.330168893167226
28-29	22.440790357126637	26.736498436017193	28.43791898043714	22.38479222641903
30-31	22.790467182351055	26.61324054456189	28.285104257904546	22.31118801518251
32-33	22.570463780481518	26.81415417057095	28.18204142093234	22.433340628015184
34-35	22.794274888911218	26.83778638461483	28.227832029698202	22.140106696775753
36-37	22.82570250086053	26.673102400585147	28.13233299333727	22.368862105217055
38-39	22.621660694398955	26.8908696047109	28.132440382664686	22.355029318225462
40-41	22.73004543905406	26.6887699516754	28.2133859151028	22.367798694167742
42-43	22.57717274548227	26.728242405556756	28.028681060558895	22.665903788402076
44-45	22.503890625302642	26.846796919439054	28.019135117058706	22.630177338199598
46-47	22.619830534185102	26.797281592133736	27.833583366354137	22.74930450732702
48-49	22.61713787038184	26.89770144011701	27.747237205005394	22.73792348449576
50-51	22.565271000711977	27.020025941178123	27.658316495814113	22.75638656229579
52-53	22.644105134319396	27.03476346485829	27.571006950669037	22.750124450153272
54-55	22.468109374387947	26.93755010316791	27.6854754500851	22.908865072359045
56-57	22.511598913857448	27.066424941513805	27.489284530571823	22.932691614056925
58-59	22.53593479481078	26.916054643339947	27.627528796059863	22.92048176578941
60-61	22.651277273247786	27.08373627283207	27.55580447713365	22.709181976786493
62-63	22.399081590035397	27.039952477826162	27.679796585616558	22.881169346521883
64-65	22.378723060779723	26.791246128500617	27.678367646127583	23.151663164592076
66-67	22.350684556588202	26.94690809256577	27.45245307464959	23.249954276196437
68-69	22.250221374833675	27.02419767953399	27.530075887462964	23.19550505816937
70-71	22.334622226417345	26.797592400843218	27.508961933542004	23.358823439197433
72-73	22.282510625630163	26.982216677828934	27.315373067206146	23.419899629334758
74-75	22.22566527142138	27.11035988600226	27.25762040473498	23.40635443784138
76-77	22.18944301261874	26.909534559863708	27.35698915218591	23.544033275331643
78-79	22.171280386580307	26.836486313041362	27.35972255343902	23.63251074693931
80-81	22.204537444058058	27.086703578738653	27.104265384297797	23.60449359290549
82-83	22.288859052826183	26.900652037913453	27.194059019512594	23.616429889747767
84-85	22.204905829598186	26.93208865063697	27.11427310513404	23.748732414630805
86-87	22.052857061261548	26.980070544332115	27.302205997592395	23.664866396813945
88-89	22.05606964629008	26.806582669790263	27.281162017398607	23.85618566652105
90-91	22.21359783128607	26.74185895288001	27.216189035406035	23.828354180427887
92-93	22.177523925710204	26.701021836656103	27.156928650332663	23.96452558730103
94-95	22.04044728792396	26.541493591992836	27.257109367462046	24.160949752621157
96-97	22.021000148506342	26.470312586282006	27.331099927612545	24.177587337599103
98-99	21.94216034598474	26.44444283696828	27.256836256851553	24.35656056019543
100	21.977346080033836	26.336116815327664	27.321742189615406	24.364794915023094
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1443.0
1	1052.5
2	928.0
3	1264.5
4	1182.0
5	1196.0
6	1815.5
7	3571.5
8	6020.0
9	7460.5
10	7039.0
11	6489.5
12	7057.0
13	7869.0
14	8750.5
15	9706.5
16	10574.0
17	11521.5
18	12618.0
19	13812.5
20	15385.5
21	17877.5
22	21280.0
23	26293.0
24	32919.5
25	41612.5
26	54306.5
27	70074.5
28	86174.0
29	105146.5
30	129800.0
31	153171.0
32	179049.0
33	210029.5
34	241385.0
35	270612.0
36	307138.5
37	341687.5
38	364387.5
39	385533.5
40	396884.5
41	404677.0
42	414145.0
43	429883.0
44	448040.5
45	465067.0
46	478303.5
47	484419.5
48	495979.5
49	501436.5
50	493420.0
51	479769.0
52	462452.5
53	449425.0
54	435535.0
55	409063.0
56	373101.0
57	332722.5
58	293746.5
59	254086.5
60	213678.5
61	177133.5
62	144084.0
63	111752.0
64	82724.5
65	61249.5
66	45457.0
67	34713.0
68	26438.0
69	19558.5
70	14824.0
71	11293.0
72	8658.5
73	6719.0
74	5007.5
75	3449.5
76	2308.0
77	1462.5
78	876.0
79	537.0
80	344.0
81	222.5
82	150.5
83	115.0
84	73.5
85	45.5
86	33.0
87	26.0
88	19.5
89	12.5
90	10.0
91	9.5
92	5.0
93	2.5
94	1.0
95	1.5
96	1.5
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030735986963135075
2	0.07883211471100386
3	0.0030751797656017347
4	4.743207864681339E-5
5	0.0013676249343164528
6	0.001106748501758979
7	7.905346441135565E-6
8	4.743207864681339E-4
9	2.3716039323406694E-5
10-11	1.9763366102838912E-5
12-13	6.324277152908452E-5
14-15	0.012051700649511169
16-17	9.763102854802423E-4
18-19	1.581069288227113E-4
20-21	2.2530237357236361E-4
22-23	0.001905188492313671
24-25	8.142506834369633E-4
26-27	0.0073638302099177785
28-29	0.008146459507590199
30-31	0.010257187007373396
32-33	0.009352024839863374
34-35	0.004347940542624561
36-37	0.003960578567008918
38-39	0.002909167490337888
40-41	0.0032886241195123947
42-43	0.001616643347212223
44-45	0.0053123928084431
46-47	0.018478747306154383
48-49	0.019490631650619736
50-51	0.014403541215749001
52-53	0.008130648814707929
54-55	0.001126511867861818
56-57	0.0024862314557371353
58-59	0.0029012621438967525
60-61	0.0034427783751145385
62-63	0.003272813426630124
64-65	0.003936862527685512
66-67	0.002747107888294609
68-69	0.006889509423449645
70-71	4.1898336138018494E-4
72-73	0.005158238552840956
74-75	0.007865819708929888
76-77	0.00709504843091917
78-79	0.004747160537901907
80-81	4.4665207392415946E-4
82-83	0.001363672261095885
84-85	9.84215631921378E-4
86-87	0.001379482953978156
88-89	0.0011778966197291993
90-91	0.001126511867861818
92-93	3.359772237482615E-4
94-95	0.0011186065214206825
96-97	0.002968457588646405
98-99	0.003466494414437945
100	0.005075232415209033
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.2649667E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.078923448860536
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.82548387491514	33.77880654427745
2	16.308570993848885	16.00814214734834
3	6.338742376091535	9.332959555147342
4	2.9959608300901364	5.881541289431138
5	1.6526342580637046	4.055475512023649
6	0.9965490547378095	2.9345732862306773
7	0.6500066381524413	2.23311382245944
8	0.45620817859937723	1.7912165019378348
9	0.2930168558705983	1.2942856654649004
>10	1.362363691355343	11.430424728091191
>50	0.0664912236374599	2.2502510037445056
>100	0.04654316360086181	4.5875541101873605
>500	0.00520581424062322	1.7350047826684245
>1k	0.002114605491576657	1.6649915962020974
>5k	2.7110326125974797E-5	0.0766926495719812
>10k+	8.133097837792438E-5	0.9449668052136843
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30194	0.23869403044364726	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25672	0.20294605383683223	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15155	0.11980552531540949	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	7.905346441135565E-6	0.0	0.0	0.0
3	0.0	7.905346441135565E-6	0.0	0.0	0.0
4	0.0	1.581069288227113E-5	0.0	0.0	1.581069288227113E-5
5	0.0	3.162138576454226E-5	0.0	0.0	1.581069288227113E-5
6	0.0	3.9526732205677824E-5	0.0	0.0	3.162138576454226E-5
7	0.0	3.9526732205677824E-5	0.0	0.0	3.162138576454226E-5
8	0.0	3.9526732205677824E-5	0.0	0.0	3.9526732205677824E-5
9	0.0	3.9526732205677824E-5	0.0	0.0	5.5337425087948954E-5
10-11	0.0	4.743207864681339E-5	0.0	0.0	5.5337425087948954E-5
12-13	0.0	5.929009830851674E-5	0.0	7.905346441135565E-6	5.5337425087948954E-5
14-15	0.0	1.1067485017589792E-4	0.0	7.905346441135565E-6	8.300613763192343E-5
16-17	0.0	1.343908894993046E-4	0.0	7.905346441135565E-6	1.1462752339646569E-4
18-19	0.0	1.383435627198724E-4	0.0	1.581069288227113E-5	1.4229623594044018E-4
20-21	0.0	1.4229623594044018E-4	0.0	7.114811797022009E-5	1.5415425560214352E-4
22-23	0.0	1.4229623594044018E-4	0.0	2.5692375933690585E-4	1.8972831458725356E-4
24-25	0.0	1.4229623594044018E-4	0.0	6.245223688497096E-4	2.2134970035179581E-4
26-27	0.0	1.4229623594044018E-4	0.0	0.0014308677058455371	2.2925504679293139E-4
28-29	0.0	1.541542556021435E-4	0.0	0.004806450636210423	2.3320772001349916E-4
30-31	0.0	1.6601227526384687E-4	0.0	0.016020184562961223	2.490184128957703E-4
32-33	0.0	1.6601227526384687E-4	0.0	0.04434108818832938	2.924978183220159E-4
34-35	0.0	2.055390074695247E-4	0.0	0.09027510368454758	2.924978183220159E-4
36-37	0.0	2.3716039323406699E-4	0.0	0.16184615768936841	2.924978183220159E-4
38-39	0.0	2.8063979866031253E-4	0.0	0.2637065465834002	2.924978183220159E-4
40-41	0.0	3.122611844248548E-4	0.0	0.3713259803597992	3.0040316476315145E-4
42-43	0.0	3.478352434099649E-4	0.0	0.48000473055931037	3.2016653086599036E-4
44-45	0.0	3.478352434099649E-4	0.0	0.6199095992013071	3.3202455052769374E-4
46-47	0.0	3.478352434099649E-4	0.0	0.7873646001906611	3.596932630716682E-4
48-49	0.0	3.596932630716682E-4	0.0	0.9613731333797166	3.794566291745071E-4
50-51	0.0	3.7155128273337157E-4	0.0	1.1422553652993395	3.794566291745071E-4
52-53	0.0	3.7155128273337157E-4	0.0	1.3231257391993005	4.1503068815961717E-4
54-55	0.0	3.7155128273337157E-4	0.0	1.5274868500490961	4.54557420365295E-4
56-57	0.0	3.8340930239507494E-4	0.0	1.765244096939469	4.5851009358586277E-4
58-59	0.0	4.0317266849791385E-4	0.0	2.028207540957402	4.7036811324756614E-4
60-61	0.0	4.347940542624561E-4	0.0	2.3012661123806657	4.743207864681339E-4
62-63	0.0	4.4269940070359163E-4	0.0	2.575427479632468	4.901314793504051E-4
64-65	0.0	4.4269940070359163E-4	0.0	2.864782922744132	4.940841525709729E-4
66-67	0.0	4.5060474714472723E-4	0.0	3.191091907794885	5.019894990121083E-4
68-69	7.905346441135565E-6	4.5060474714472723E-4	0.0	3.554899903689164	5.059421722326761E-4
70-71	7.905346441135565E-6	4.54557420365295E-4	0.0	3.9416215462430753	5.257055383355151E-4
72-73	7.905346441135565E-6	4.5851009358586277E-4	0.0	4.334612128524807	6.324277152908452E-4
74-75	7.905346441135565E-6	4.5851009358586277E-4	0.0	4.733013920445495	6.324277152908452E-4
76-77	7.905346441135565E-6	4.5851009358586277E-4	0.0	5.156614004147303	6.363803885114129E-4
78-79	7.905346441135565E-6	4.5851009358586277E-4	0.0	5.625646113846317	6.442857349525486E-4
80-81	7.905346441135565E-6	4.664154400269983E-4	0.0	6.1247699247735135	6.561437546142519E-4
82-83	7.905346441135565E-6	4.743207864681339E-4	0.0	6.629352377418314	6.877651403787942E-4
84-85	7.905346441135565E-6	4.743207864681339E-4	0.0	7.131001946533454	6.91717813599362E-4
86-87	7.905346441135565E-6	4.822261329092695E-4	0.0	7.651177695033395	7.035758332610653E-4
88	7.905346441135565E-6	4.901314793504051E-4	0.0	8.060085692374352	7.035758332610653E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	21385	0.0	45.404488	1
GTATCAA	35815	0.0	37.067253	1
TCAACGC	43060	0.0	30.241783	4
CAACGCA	43755	0.0	29.750687	5
ATCAACG	43955	0.0	29.605562	3
AACGCAG	45505	0.0	28.626642	6
TATCAAC	46015	0.0	28.465418	2
GTACATG	44890	0.0	27.435846	1
TACATGG	46645	0.0	25.963543	2
ACGCAGA	51895	0.0	25.065332	7
ACATGGG	47595	0.0	24.625992	3
CGCAGAG	53585	0.0	24.21029	8
CATGGGG	31025	0.0	22.296715	4
GCAGAGT	60365	0.0	21.350742	9
GAGTACT	31235	0.0	20.706995	12-13
AGAGTAC	51510	0.0	19.820257	10-11
GTACTTT	32990	0.0	19.318172	14-15
ATGGGGG	17010	0.0	19.311577	5
CAGAGTA	58730	0.0	18.828281	10-11
AGTACTT	34245	0.0	17.740814	12-13
>>END_MODULE