FastQCFastQC Report
Mon 6 Jun 2016
ERR523188_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523188_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12642826
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT434590.34374434956235256No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT351550.27806283183838804No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT247080.1954309898752067No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221420.17513489468256543No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131400.10393245940425028No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG206300.053.3196832
GTACCTG236100.046.8448141
ACCTGGG247200.043.8130763
CCTGGGG227250.031.5971984
TATAACG12050.031.2219142
GTACATG513550.025.41141
TAACGCA15900.025.1382624
TACATGG533750.024.0801012
CATGGGG329100.023.7045634
ACATGGG535500.023.4218853
ATAACGC16850.022.8861243
CTGGGGG142450.021.620025
ATGGGGG191550.021.0857125
GTATCAA718650.020.947751
GAGTACT378550.020.14052212-13
GGTATCA560200.019.58351
TGGGGGG196350.019.3144366
GTACCCG22750.019.2312011
GTATAGG37150.018.7416481
TCAACGC799300.018.466944
Produced by FastQC (version 0.11.5)