FastQCFastQC Report
Mon 6 Jun 2016
ERR523188_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523188_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12642826
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT388960.3076527352349862No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT342970.2712763744434986No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT255320.20194852005398162No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217780.17225579154533963No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG203950.052.907462
GTACCTG236150.046.411891
ACCTGGG239450.044.4321863
CCTGGGG216100.033.1193054
TATAACG12150.029.01042
CTGGGGG133000.025.333895
GTACATG527200.023.7771421
TACATGG546650.022.5505772
ACATGGG541350.022.144633
CATGGGG331150.022.1378754
ATAACGC15600.021.9904613
TAACGCA15850.021.6436084
TGGGGGG177500.021.1799186
TATCACG12850.020.4811152
ATGGGGG186750.020.4580525
GAGTACT389700.019.62641512-13
GTATCAA739450.019.5211071
GTACCCG22950.019.463271
GGTATCA585500.018.9441851
GTACTTT432800.017.62051414-15
Produced by FastQC (version 0.11.5)