FastQCFastQC Report
Mon 6 Jun 2016
ERR523187_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523187_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6152349
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235830.38331700623615467No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192270.31251478093976787No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114640.18633533305734118No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61830.10049819995582175No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102000.058.4394531
GTATCAA186900.041.609571
TACCTGG66450.037.4886632
TCAACGC235400.032.9196664
ATCAACG238250.032.4088443
CAACGCA240050.032.281195
TATCAAC241850.032.1057622
ACCTGGG76750.031.9632593
AACGCAG248900.031.3918296
GTACCTG81600.030.960921
GTACATG212700.027.6485421
ACGCAGA282850.027.5570287
CGCAGAG292150.026.6937148
TACATGG222650.025.9235672
GTGGTAT48050.025.0654931
ACATGGG225550.024.8782843
GCAGAGT321450.024.0696229
CATGGGG161550.023.9114594
TGGTATC50250.022.5423952
TATAACG6050.022.5300252
Produced by FastQC (version 0.11.5)