FastQCFastQC Report
Mon 6 Jun 2016
ERR523187_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523187_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6152349
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216970.352662048268068No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186250.30272990040064374No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110750.18001254480199352No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99800.057.549661
GTATCAA185400.041.7109151
TACCTGG64650.038.045742
TCAACGC230900.033.032594
ACCTGGG73200.032.8724143
ATCAACG234600.032.4533653
CAACGCA236250.032.2646565
TATCAAC237700.032.2105452
AACGCAG247150.031.0313386
TATCACG5500.030.7831232
GTACCTG82050.030.442031
TATAACG6100.027.7552762
ACGCAGA283600.026.960157
CGCAGAG292700.026.04748
GTACATG208350.025.5118871
GTGGTAT46250.025.2230511
TACATGG216800.023.9704322
ATAACGC7900.023.7960933
GCAGAGT325400.023.2565129
ACATGGG216550.022.9614983
Produced by FastQC (version 0.11.5)