FastQCFastQC Report
Mon 6 Jun 2016
ERR523186_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523186_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8934922
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT621170.6952159179453385No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276470.3094263162006339No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207680.23243627644427114No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT185610.20773544525626522No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC138990.15555815708296056No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC129720.14518313646162775No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122320.13690102722776987No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA103380.11570330440489576No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG101840.11397973032109289No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG339000.026.4604021
TACATGG337800.025.885492
ACATGGG340250.025.036323
CATGGGG224250.022.2829254
GAGTACT243300.021.23161112-13
AGAGTAC389250.019.69060310-11
GTACTTT262550.019.61267914-15
GAGTACA254350.019.2968541
ATGGGGG135400.018.7134425
CATGGGA124950.018.2087654
AGTACTT262050.018.16164212-13
AGTACAT253050.017.9091012
TACCTGG66050.016.299182
ACTTTTT320400.016.2910916-17
CGCCGTA8600.015.85205494
TACTTTT307000.015.29617314-15
GTATTAG26500.014.9022261
CCTATAC20100.014.0334873
GTATAAA51400.013.9941361
ATGGGGA98200.013.9783185
Produced by FastQC (version 0.11.5)