FastQCFastQC Report
Mon 6 Jun 2016
ERR523186_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523186_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8934922
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT622650.69687233979211No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253140.2833152880349711No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201630.22566509254361705No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT183060.20488147518243582No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC144080.16125490519111413No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC135290.1514171024660316No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114100.12770117075448448No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA103110.11540111933825499No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA89930.10065001127038378No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG332850.026.7499331
TACATGG335800.025.978932
ACATGGG342250.024.7710343
CATGGGG222050.022.3704784
GAGTACT247650.020.9282212-13
AGAGTAC401800.019.3398810-11
GTACTTT266400.019.32862314-15
ATGGGGG131500.018.6190785
AGTACTT267700.018.04369512-13
CATGGGA125400.017.1265434
ACTTTTT317400.016.29437816-17
GAGTACA252300.016.1070921
AGTACAT248500.015.2451832
TACTTTT309700.015.20720114-15
TACCTGG66250.014.6861672
GTATTAG26700.014.0928671
GTGTAGC48550.014.0475111
ATGGGGA95650.013.9042535
GTATAGG25200.013.6252011
TATACTG37100.013.4269745
Produced by FastQC (version 0.11.5)